BtaINT0120961 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018928 | QARS
Description
glutamine--tRNA ligase [Source:RefSeq peptide;Acc:NP_001029640]
Coordinates
chr22:51485280-51485632:+
Coord C1 exon
chr22:51485280-51485355
Coord A exon
chr22:51485356-51485567
Coord C2 exon
chr22:51485568-51485632
Length
212 bp
Sequences
Splice sites
5' ss Seq
TGGGTGAGC
5' ss Score
7.23
3' ss Seq
CCTTCTGTCCATGTTTCTAGGAG
3' ss Score
10.33
Exon sequences
Seq C1 exon
GTGGAGGCTGCCATAAATCGCCACCGAGCCAAGCTCCTGGTAGAGCGTTACCATTTCAGCATGGGGCTGCTGATGG
Seq A exon
GTGAGCAGGGCCTAGGGCAAGGGACTATGTTCTTCTCGCTGGACATGCCTTTTCTTCTGGGGATGAGTGAGAGGGAGGTGGGAGGAGGAATAGGAGCACCTTAGAAGTGGTCTCGCTCTGGTTTCTGAGGTCAGCCAGGCCCTTTTCTTGGGAAAGAGGATGGGAAATGTCCTGCTTTGCCTTTTTGCATCTCCTTCTGTCCATGTTTCTAG
Seq C2 exon
GAGAGGCTCGGGCTGCACTCAAGTGGGCAGATGGCAAAATGATCAAGCATGAAGTAGACATGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018928:ENSBTAT00000025191:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0455810=tRNA_synt_1c_R1=FE(15.3=100)
A:
NA
C2:
PF0455810=tRNA_synt_1c_R1=PD(7.4=54.5),PF0455710=tRNA_synt_1c_R2=PU(8.6=36.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGGAGGCTGCCATAAATCGC
R:
TACTTCATGCTTGATCATTTTGCCA
Band lengths:
132-344
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]