BtaINT0120963 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018928 | QARS
Description
glutamine--tRNA ligase [Source:RefSeq peptide;Acc:NP_001029640]
Coordinates
chr22:51486185-51486424:+
Coord C1 exon
chr22:51486185-51486238
Coord A exon
chr22:51486239-51486363
Coord C2 exon
chr22:51486364-51486424
Length
125 bp
Sequences
Splice sites
5' ss Seq
AAGGTGGGA
5' ss Score
6.03
3' ss Seq
AACTTTTGAACTTCTCTCAGGTG
3' ss Score
6.95
Exon sequences
Seq C1 exon
GTCCTCCATCTTCTGGGTCCCAAGACAGAGACTGATCTGGAGAAGAAGCCCAAG
Seq A exon
GTGGGACTGAGCATGGTCTAGGGTCCCTGGGGAGGAGGTAGGGTAGGGTGAAATTTCAGTTTGGGGAAGGGGGTATCTGAATCCAATAATCCTAAGACCTTGACCAACTTTTGAACTTCTCTCAG
Seq C2 exon
GTGGCAAAGGCTCGGCCAGAAGAGACAGACCAAAGGACAGCAAAGGATGTGGTGGAGAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018928:ENSBTAT00000025191:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.556 A=NA C2=0.810
Domain overlap (PFAM):
C1:
PF0455710=tRNA_synt_1c_R2=FE(18.3=100)
A:
NA
C2:
PF0455710=tRNA_synt_1c_R2=FE(21.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCCTCCATCTTCTGGGTCCC
R:
ATTCTCCACCACATCCTTTGCT
Band lengths:
114-239
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]