BtaINT0128802 @ bosTau6
Intron Retention
Gene
ENSBTAG00000032140 | SBK1
Description
SH3 domain binding kinase 1 [Source:HGNC Symbol;Acc:HGNC:17699]
Coordinates
chr25:26077327-26079131:+
Coord C1 exon
chr25:26077327-26077530
Coord A exon
chr25:26077531-26078610
Coord C2 exon
chr25:26078611-26079131
Length
1080 bp
Sequences
Splice sites
5' ss Seq
CAGGTACCT
5' ss Score
8.16
3' ss Seq
CCCTTCCTCTCCGGCCGCAGGTG
3' ss Score
11.21
Exon sequences
Seq C1 exon
GGCACGAAGATGGCGCTGAAGTTTGTGAACAAGAGCAAAACCAAGCTGAAGAACTTCCTGCGGGAGGTGAGCATTACCAACAGCCTCTCCTCCAGCCCCTTCATCATCAAGGTCTTCGACGTCGTGTTTGAGACCGAAGACTGCTATGTCTTCGCCCAGGAGTACGCGCCCGCCGGGGACCTATTCGACATTATTCCTCCTCAG
Seq A exon
GTACCTGGGTGGTGGCTGAGGGAGGGGAGAGGCTCTTCTGGGTCCCTGAGTGTGAGAGCTGAGGAGGAGTCAGGCCCCTCCCTTAAATCATTTATTAGGCGCCTACTGTATATCAGCCATTGTTTGGAGGGCTGCGGATACACAGTGAACAAAAGGTCTTGCTATGGGCCTAGTGGGAGAAACAGACCCTCCACAGTTAGCCAGATGAGGCATTAAGATGATGGAGAAGGGAATGGCAACCCACTCCAGTATTCTTGCCTGGAAAATCCCAGGGACAGAGGAGCCTGGCCTGCTACAGTTCACAGGTTCGCAAAGAGTCAGACACGACTGAAGTGACTTAGGCACCGCACTGCAATGAGGACCAGCCGGTGGGCGGGATGAAGTCCAGCAGCCCCCTCCTCCACTGCTGCATAGGAACAGGTGGGGACCCAAGAGGGGAAGTGGCAGGACCACCAGCCTTCCCAGAGGAGGTGCACGGGACGGGGAGACTGCCCCCGGGGATTCCCGGCACCTGGCTTTGCAGTCGGCCAGGATGAAACACTGCCCTGCCGCTCACCGGATAAGTGACAAGGCCTCTCAGAGCCGCCGATTAAGGGAGATTAGGTATACAGTAGACCATGCGGGATAGGGTCCTTATTAGCCCAGTACTTGGCCCAAAGCAGGCGCTCCGTACAGGTACTGGGGGAAAAGCTGGGGAAGGTTCGAGGGGTGTGTGGGGGCCAGGGAGGGAGCCGAGGATGCCCAAGTAGGATAAGGAAGGGCGGTTGGCCCCGGATCTGCCCAGGAAAGGGACCCCGCAAGGAGTTCGGGTAGAGGGACAGGGCGGGGGCGGGGATGGGAAGGAGAGGGCGGGACGGGGAGTGGGCGGGGATGGGAAGGAGGGGGCGGTTGGGGGGCGGGGATGGGGAGGAGAGGGGCGGGGGGGCGGGGCGGGGGTGGAAGGAGGGGGCGGGGCGGGCGGGGCGGGGACTGGGCGGGGATGGGAAGGAGGGGGCAGTTGCGGGGCGGGGATGGGAAGGAAGGGGCGGGGTGGTACGTGCCCGCGCCCCTGAGCCGCCCTCCCTTCCTCTCCGGCCGCAG
Seq C2 exon
GTGGGGCTCCCCGAGGACACCGTGAAGCGCTGCGTGCAGCAGCTGGGCCTGGCGCTGGACTTCATGCACGGTCGGCAGCTGGTGCACCGCGACATCAAGCCGGAGAACGTGCTGCTGTTCGACCGCGAGTGCCGGCGCGTGAAGCTGGCCGACTTCGGCATGACGCGCCGCGTGGGCTGCCGCGTGAAGCGGGTGAGCGGCACCATCCCATACACAGCGCCCGAGGTGTGCCAGGCGGGCCGCGCCGACGGCTTCGCGGTGGACACTGGCGTGGATGTGTGGGCCTTCGGCGTGCTCATCTTCTGCGTGCTCACCGGCAACTTCCCGTGGGAGGCGGCGTCGGGCGCCGACGCCTTTTTCGAGGAGTTCGTGCGCTGGCAGCGGGGCCGCCTGCCGGGGCTGCCGTCGCAGTGGCGCCGCTTCACCGAGCCAGCGCTGCGCATGTTCCAGCGGCTCCTGGCGCTGGAGCCCGAGCGCCGCGGGCCGGCCAAGGAGGTCTTCCGCTTCCTCAAGCACGAGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032140:ENSBTAT00000063806:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=PU(28.2=98.5)
A:
NA
C2:
PF0006920=Pkinase=PD(71.0=97.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TACCAACAGCCTCTCCTCCAG
R:
GCTGTGTATGGGATGGTGCC
Band lengths:
348-1428
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]