BtaINT0155994 @ bosTau6
Intron Retention
Gene
ENSBTAG00000012335 | UBA7
Description
Bos taurus ubiquitin-like modifier activating enzyme 7 (UBA7), mRNA. [Source:RefSeq mRNA;Acc:NM_001012284]
Coordinates
chr22:50998308-51000135:+
Coord C1 exon
chr22:50998308-50998499
Coord A exon
chr22:50998500-51000042
Coord C2 exon
chr22:51000043-51000135
Length
1543 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
TCCTCACTGCCACCCCCCAGTTC
3' ss Score
6.2
Exon sequences
Seq C1 exon
ACCAAGCGAATTGTGGGCCGGATTATCCCAGCCGTTGTCACCACTACAGCGGCCGTGGCTGGCCTGGTGGGCCTGGAGCTGTACAAGGTGGTGGGTGGACCACGGCCCCGCCATGCCTTTCGCCACAGCTATCTGCACCTGGCTGAAAACTACTTTAGCCGCTGGGTACCTAAGGCCCCAGACATCCAGAAG
Seq A exon
GTGAGCCCTGGACACCCCATCTGTACCAGACCTGAGTTTTCCCTGCACCTACCCAAACAGGCCCTACTCCAGCTTTAGGCCCTCGTGGGACGCAGACTCTGGGGGAGTCCTCAAGACTTCCTTCAGCTGCCTCCCTGCTGTACAGCCAGGCTGGGACCTGTCAGACACAGGAAGCAGCCGTCAGCCACCCCCAACCCCCAACCCTCCGGGTCTGGGGGAGCTGCAGCTTTAACTCATTAGTGGAGCCAGACATCCATCCCCCACCATCCCCGCCTTCCCTGAGGGGGAGGGGGGACTAGGGCCCAAGCCCTAAACAGAGCCTAACGTCAGGCTGCCAGGCAGGGGGATCCTCGCCCGCCCCCCTCAACTGAGTTAAAGGGCATTGAACTCACCAGCATGGGTGTGGGGGTGTGCAACCCTGTCACAGGGTGTGAGGGCACCCGGGTCTGGGCATCCCTGGCCCCATGGTGTGTGGCCATGGAGGAAGCATGCTTGCACATCTGTGTGCCAGAGGAGACTGCAGGGCCCTTTCATGATGTGGGCCTGCATGGTTTTGCCTGTGAGTACACGTGTGGGCTTGTGTGCATATGTGTTCCTTCAAGAGTTGGGCTGTGAATGCCCCAGCTGTGTACCCAGGCTCTGCAAAAGTTGGCATTTCCTTGGGGACACAGGAGAGGGTCTGGAGAGAATTCTCTCACCAGGGCAGAGGGGAGAGGTCATGGGCCGGCACTGGGCTTAGGCTGAGCAGCCCTGGGGGTCCCTCAGGGCACAGCACACAGAGCCCCTTTGTGAGGGCCCCTCCTCTAGCTAGAACACAAGCTGCCTTTGTCCATGGAGGAGGGGGAAGGGAGGGGAAGGGTCCCCCACATCACTTCTGGTCCTGTCTCAGCTTGACCTCGCTGTTTTCTGGGGAGCTGGCAGAACCCCTCTAGTCTTGGTCCCTGGCTGCCTGCCACCCTTATGCCCTCAGCTCTGGGTACCAGAACCCCAAGACTCAAGAAACAAGGAACAAAGGGACAGGAAGAGAGTCCTCTGTGGACTGTGTTCCCGCTGGAGTCACTGTGCCCCTCACCCATGTGTCTATGAGAAAGTCCAAGGCCCTGGTGGTAAAGAATTAGGGGACCGGATGTACACGCCCCCCCACCCTCTCTTCCAGCCTTGCATTTGAGGAACAGGCAGCTTTATCAGAGATTGCAGCAGCCCTGCTCCTGCTTCCTCGCTTCCTCAGGAAGCATTGCCCCCCCCTCCAACACACACACACACACACACACACACACACACACAATCAGCCTGTGTGATAGATCGCCTGTTTCCCTGTGCAGGGGGCCCCATAGCCCCATTTCCTGTCCCAGCACAGGCAGGGAGGGTGTTGAGATTCTGGGCCTAGATCCCACGTTTCATCCCCACCCCCTGGCTTCTGCTTCCTGCCTCTAGTCCAGCTCCTCCCCTAGGAAAGGGGCCATAAGCTATTGGCAACTGGGGCAGGGAGAGGGTGTGTGTGTGTGTAAGAAGCTTCTCACTCCTCCTCACTGCCACCCCCCAG
Seq C2 exon
TTCCATCACCTGAAGTGGACCTGCTGGGACCGCCTGGAAGTGCCTGCTGGGCAGCCTGAGAGGACCCTGGAGTCGTTGCTGGCCCACATCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012335:ENSBTAT00000016365:21
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0213416=UBACT=PD(47.8=51.6),PF093585=UBA_e1_C=PU(19.7=37.5)
A:
NA
C2:
PF093585=UBA_e1_C=FE(24.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGAATTGTGGGCCGGATTAT
R:
CTGGATGTGGGCCAGCAAC
Band lengths:
280-1823
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]