BtaINT0166075 @ bosTau6
Intron Retention
Gene
ENSBTAG00000002810 | ZNF777
Description
zinc finger protein 777 [Source:HGNC Symbol;Acc:HGNC:22213]
Coordinates
chr4:113259309-113261531:-
Coord C1 exon
chr4:113260686-113261531
Coord A exon
chr4:113259436-113260685
Coord C2 exon
chr4:113259309-113259435
Length
1250 bp
Sequences
Splice sites
5' ss Seq
AAGGTAACC
5' ss Score
9.24
3' ss Seq
AGCCTATTGTGTGGTTTCAGGTC
3' ss Score
8.4
Exon sequences
Seq C1 exon
ATGGAGACCCAACGCTCCTCCCCTCTGTCATTCCCCAACGTCCTGCAGGAAGAAACTCCACGTCAGGTCCCGGCCGGACTCCCTCGAGAAACTCTGTTCCAATCCCACGTGCTTCCCCCCAAAGAACTCCCTTCTTTGTCTCCCGCTGTCTCCCGGCAAGGCTGCCTACCCCAGACTCCTGGCGCCCCCAAGCAAGAGACTTCTGGCCGAATGCCGCCTGTGCTCCAGAAGGGACCCTCACTCCTGTATCCTGGCGCTCCCGAGCAAGAGACTCGGATCCAGGGCCCCCTGACTTCCCCAGAAGAGCCCCCATATCCGCCCCCTGCCGCTGCCGAACAAGAGATGTCCCTTCTCTCCCACTCCGCCCACCATCAAGAAGCCCCGCTCCACTCCCCCGAAGTTCCTGAGAGAGACCCCCTGACCCTCTCCCCCACCGTGCCCGAGACTGACATGGACCCCCTGCTCCAGAGCCCAGTTTCCCAAAAGGACACCCCCTTCCAGATCTCTGCTGCGGCCCAGAAGGACACCCCACTCCCAACGGCAGAGATCACCCGCTTGGCCGTGTGGGCTGCCGTCCAAGCGGTGGAGAGGAAACTGGAGGCCCAAGCCATGCGGCTCCTGACCCTGGAGGGCAGGACCGGCACAAATGAGAAGAAAATAGCCGACTGCGAGAAGACAGCCGTGGAGTTCGCCAACCACCTGGAGAGCAAGTGGGTCGTGCTGGGGACCCTGCTGCAGGAATACGGGCTGCTGCAGAGGCGGCTGGAGAACATGGAGAACCTGCTGAAGAACAGAAACTTTTGGATCCTGCGGCTGCCCCCGGGCAGCAACGGGGAAGTTCCCAAG
Seq A exon
GTAACCTTATCCCAGCCAGGGCTTCCTAAGGAGTGACCGGGACTGGAATGGATTTTAGGAATTTAGTATCAAAGAAAGGGAAAGCCGTAGGTAAGAAGGAAAAGAATCTGATAATGTCTGGTCCCCAACTCCACTAAGAACAGGACACACCCATACCAAATGATTCCTCCTGTTGATGAAAAGCATAGGTTATAGATCAGAGGGACGTTCTAGTGACGGGAGCTGTCAGGGTAGAAGGAAGTTCATTCGTGACATCTGGGGATAAAAGGCGCATTGAAGGCCAAGTGGCTGAGCTGTGGACAAGTGTGGACCAGTGTGGTTTCCCGTGGGGGTGGAAGGAAGACTGTGAAATGTTACAGGGCCCTGTTGCTTGCAACATTTGTATCAGTTAACTTAAAGTCATGTTAATGCCAGTAAAAATGTGATTTTCAGAGATGTTCTAAAACACCAGAGGACAGTTAGGTTTTAATATTGTTTCCCAGAGGAGAACACTTGAAAGTGGACTGGTTTTCTTTCTGTTTTTTGTTTTTTATACTACTGCCTCTCTGCGCAGCCTCAAGGCAGGGCCGGGGTGGGGACAGGGGACGATGCATGGACCGTGTTGTCTAGGCCAGCGCTTTGCAGTCGTGTTGTGTATATGAATCACCTGGAAGTGTGTGTCTTAGTCGCTCAGTCATGTCCAGCTCTTTACCACCCCAAGGACTGTGGCCTCCCGGGCCTCCTCTGTCTGGGATTCTCCAGACAAGAATACTGGAGTGGGTTGCCATTCCCTTCAGGGGATCTTCCCGAGCCAGGGATTGAACCCAGGTCTCCTGCATTGCAGGCAGATTCTTTACCATCTGAGCCACCAGGGTAAGTGGCTGAGTCACCTGGGGATCTTACTAAATGCCGAGTCTGAATCAGAGGGTCTGGAGTAAAGCCCGAGAGTCTGCATTTCCAGCATGTTCTCCGGTGGTGCCACTGCTGGTCTGCAGACCACGCTTTACAGCAGCAGGGATTATCCTGTTAGAGGAGAAGGGGACCAGACAGCCCGGGGCCCTGACTGTCTCGGGCCAGGACAGTGGCTGAAGCCTTCTGATTTCTAGAGAGGTCCCTGGTGAAAACTCATAGCTGGGCCTTTTCTGCTTCAGGCCTGAGAGTTTTTACCCAAGCCTAGGGGGCCAGAGCTGTTCTCGGCTTTGGGATCCGGCAGCCCCAGGCCCAGTGACGCCTTCCAGTGGGTCCACACTGAGCCTATTGTGTGGTTTCAG
Seq C2 exon
GTCCCTGTGACATTCGACGACGTGGCCGTCCATTTCTCGGAGCAGGAGTGGGGGAACCTGTCCGAGTGGCAGAAGGAGCTGTACAAGAATGTGATGCGCGGCAACTACGAGTCTCTGGTTTCCATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000002810:ENSBTAT00000003635:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.677 A=NA C2=0.023
Domain overlap (PFAM):
C1:
PF124173=DUF3669=WD(100=28.4)
A:
NA
C2:
PF0135222=KRAB=WD(100=95.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAGCGGTGGAGAGGAAACTG
R:
TGTACAGCTCCTTCTGCCACT
Band lengths:
355-1605
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]