Special

MmuINT0179848 @ mm9

Intron Retention

Gene
ENSMUSG00000071477 | Zfp777
Description
zinc finger protein 777 [Source:MGI Symbol;Acc:MGI:1919556]
Coordinates
chr6:47992028-47994700:-
Coord C1 exon
chr6:47993843-47994700
Coord A exon
chr6:47992155-47993842
Coord C2 exon
chr6:47992028-47992154
Length
1688 bp
Sequences
Splice sites
5' ss Seq
AAGGTACAC
5' ss Score
7.56
3' ss Seq
ATGCAGTTGTTTGGTTTCAGGTT
3' ss Score
6.75
Exon sequences
Seq C1 exon
GTCCAGCAGCTGGACATGGAGGACGATCGCTCGTCACCACTGTCATTCTCCAGTGTCCCACACGAAGAGTCTGTGCGTCAGGCCCCTGCTCGACTCCCTAGAGAAACTGTGTTCCCTTCTTGCATTCTTCCTCCAAAAGAAGTTCCTCCTTTGTCTCCCACTGCCTCCCGGCAAGGTGTCTTGCCCCAGACTGACAGTACTACTAAGCAAGAGACATCCAGCCAGATGTCACATGTTCTTCAGAAAGGACCTTCACTCCTGTATCCTGCCACTTCTGAGCAAGACACACCTCACCAGGTATCCCTTGCTTCCCAGGAAGAAACCCAGTGCTCTCCTTCAGCAGCTGCTCAGGGGATCCCCTTCCTCTCGCACTCCGCCCACCGTCAGGAAGCTCCACTCCACTCTCCTGAGGTTCCTGAGAAAGACTCGCTGACTCTCTCACCCACAGTTCCCGAGACCGACATGGACCCCTTGCTCCAGAGTCCCACTTTGCAAAAGGACACTCCCTTCCATACCTCTTCTGCCGCCCAGAAAGAACAGCCACTGCCCACTGCAGAGATCACTCGATTGGCTGTGTGGGCTGCTGTCCAAGCAGTGGAGAGGAAACTGGAGGCCCAGGCCATGCGACTACTGACTCTGGAGGGCAGGACAGGGACAAATGAGAAGAAGATAGCAGACTGTGAGAGAACGGCTGTGGAGTTCGCAAATCATCTGGAGAGCAAGTGGGTTGTACTGGGGACCTTACTGCAGGAGTATGGGCTGCTGCAGCGGCGCCTGGAGAACATGGAGAATCTGCTGAAAAACAGAAACTTCTGGATCCTGCGGCTGCCCCCAGGCAGCAATGGAGAAGTTCCCAAG
Seq A exon
GTACACACATTTCATTGGGGTCTTCCAGAGGCATCTACAGGGAACGTGGTTTAGGGATTCTGTCAGTTGAAAGAAAGTGAAAGTGGTAGTTGGGAAAGGAAAAACAAGAAACAAGTGTCCCTGCCTTCCAGTATTGCTTCACCGAGATCAGGGCACACCAAAAGCAAATTATCTTTCCTGTTGGTAAAAGCAAAAACTGGCAGAAAAGTCCATTTAGAATGGGGTTAGAAAAGCTCTTCCCAGAGCCTGAGATAGGATGCAGAGAGCTTACACAGAGCAGGTCCTGCCAGTCTGCCCTCCACACTCTGATCTGCATGGTGGTTTCTGACCACTCTGTTACTATTGTGTGGCCGGCATTTGCTACCTGTGGGTTCTTTATTCTTCTACCCTTCCCCACCCTTTCAACCTCTAACACTAGATAGGAGAGAAAAAAAGGATAGAGGGGAGAGAGGGCGATGTTATTGTTAGACTACTTCCTGCTGTTAGGGTTATTGAGTTCCTTGGGGCAAGTTTGATCATCTTCACTATCAGGATAGCTAATTCTTGTTTCTTCTTTACTCAACTTTTTAACAAACTGAGACCAACAACAACCAACACCCTGCCCCTCGGAGCTAGCATTTATATGCCCTATGAAAGATTTCCAGAACTTCAAACTCCACACAGTTGCTGTCACTAGCAAATCACACCCCTGCACGAGGCAAATCATAGTCAGCTGCTGTGGACAGCCTGAAGCAGCCCCATAGTCCACACCTGGGATTAAAATGAAATCACAGTCCGATTTCTGTGGGTTTGTTTAAAAGAAACCAAAATCCTCACTACAATACTGGGAAAAAAGAAAATTATGTCTACACTGAAAATGAACCCTTTTTCTAGCCGTCCGTCCCCTGAAGAGTAGATCACAAGTGCACCCAGTATTCACCTGAGGGGGCATTGTGTTCCATCATTGGATGGGGTAAAGTGCTGGAGGTTTTGTATAGGTTTTATGCAAAAATCTGTGCCGTTTTATACATAAAGACTTCAGCATCTGCCGACTTTGGTATTCTGAGGGTTCTGGAGCCCATTCTTTGCAGGTACTTAGGGCCCTATGGGCAGTGCTTAATGGTTTACCATCAGACTGGATAGGGAGAAGAATATAGTTTAACTCTTAGGTTTTATTCTGTTTAATCTTTTTGTCAATTTACTTATAAATCCTGAATTTCAGTAAAATTGATTTTAGGAAATATCCTTAAGGAGCTAAGAAATGGATTTTAATCTTATTTTCTAGAAGGTTCTAAAGGAACATGAGTGTGGACCAGTTTTCTTTTTATTCCAAACAGACTGAGTCTGTGTATCCGCCAGTTTCTCTTGAAAGCTGTTGTGCATTAGAACTCCAGGCTCTCACTAAAGGCTTACTGATCTCTGAAGTTCCGGGGTGAGGCTTAAGAGTCTGCACTTGCAGCAGGTTTCCTGGTGTTCCTGGTGCTCTGTGTTTTAAAGCTGGGGAAGAGCCCAGGCAAGATGAGTCCTCCACTCCTTGGCCAGGGATGTGGCCAGCAGAATGTTGATAAGGCTCCCTACAACACCCCCCACCACCAGCAAGTGCAGGGGAGGAAGGTTTCCCTCTCTAGTCCTGAATAGAGGGAGTTGGGGTTTGGTCGTCTATGGCATTAACCACTTCGGTCTTTCCTGATGCAGTTGTTTGGTTTCAG
Seq C2 exon
GTTCCTGTCACATTCGATGATGTCGCCGTCCATTTCTCTGAGCAGGAATGGGGAAATCTCTCTGAGTGGCAGAAGGAGCTCTACAAGAATGTAATGAGGGGCAACTATGAGTCTCTGGTCTCTATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000071477-Zfp777:NM_001081382:2
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.646 A=NA C2=0.023
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF124173=DUF3669=WD(100=28.5)

							
A:
NA

							
C2:
PF0135222=KRAB=PU(95.1=90.7)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000110230





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000093637, ENSMUSP00000110230f5436A, ENSMUSP00000116303, ENSMUSP00000117227, ENSMUSP00000123597
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr7:149454238-149455176 
LOW PSI
HsaINT0188359
(ZNF777)
Human
(hg19)
chr7:149151329-149152267 
LOW PSI
HsaINT0188359
(ZNF777)
Rat
(rn6)
chr4:77704378-77706136 
LOW PSI
RnoINT0168644
(Zfp777)
Cow
(bosTau6)
chr4:113259436-113260685 
LOW PSI
BtaINT0166075
(ZNF777)
Chicken
(galGal4)
chr2:539700-541630 
ALTERNATIVE
GgaINT1003914
([1])
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ATCACTCGATTGGCTGTGTGG

				
R: 
CTCAGAGAAATGGACGGCGAC

				
Band lengths: 
342-2030

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"