DmeEX0000137 @ dm6
Exon Skipping
Gene
FBgn0000667 | Actn
Description
The gene alpha actinin is referred to in FlyBase by the symbol DmelActn (CG4376, FBgn0000667). It is a protein_coding_gene from Dmel. It has 10 annotated transcripts and 10 polypeptides (4 unique). Gene sequence location is X:2023822..2042311. Its molecular function is described by: protein binding; actin filament binding; calcium ion binding; actin binding. It is involved in the biological process described with: sarcomere organization; actin filament bundle assembly; actin cytoskeleton reorganization. 48 alleles are reported. The phenotypes of these alleles manifest in: non-connected functional system; larva; somatic muscle; sensory system; pericardial cell. The phenotypic classes of alleles include: increased mortality; phenotype; some die during pupal stage; visible. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, during late pupal stages, in stages of adults of both sexes.
Coordinates
chrX:2027935-2030368:-
Coord C1 exon
chrX:2030215-2030368
Coord A exon
chrX:2028665-2028750
Coord C2 exon
chrX:2027935-2028027
Length
86 bp
Sequences
Splice sites
3' ss Seq
CAACACGCACACGTTCACAGATC
3' ss Score
4.48
5' ss Seq
AAGGTCTGT
5' ss Score
6.43
Exon sequences
Seq C1 exon
GATGGTCTGGCCTTCTGCGCCCTTATCCATCGCCATCGTCCAGATCTAATCGACTACGCCAAGCTGTCCAAAGACAATCCTCTGGAGAATCTTAATACTGCCTTCGATGTGGCCGAGAAGTACCTGGATATTCCGCGCATGTTGGATCCAGATG
Seq A exon
ATCTCCAGAATACAGCTTTGCCCGATGAGCGGGCGGTGATGACGTATGTGTCCTCGTACTATCACTGCTTCAGTGGCGCCCAAAAG
Seq C2 exon
GCGGAAACCGCTGCCAACCGGATCTGCAAGGTGCTCAAGGTCAACCAGGAGAACGAACGCCTCATGGAGGAGTACGAGCGTCTGGCCAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0000667_MULTIEX1-2/2=C1-C2
Average complexity
ME(1-2[100=100])
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (disopred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0030726=CH=FE(49.0=100)
A:
PF0030726=CH=PD(23.1=82.8)
C2:
PF0043516=Spectrin=PU(9.9=35.5)
Main Inclusion Isoform:
FBpp0302777

Main Skipping Isoform:
FBpp0070331

Other Inclusion Isoforms:
FBpp0070330
Other Skipping Isoforms:
FBpp0070329, FBpp0292167, FBpp0302778, FBpp0302779, FBpp0305935, FBpp0305936
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTACGCCAAGCTGTCCAAAG
R:
CTCCATGAGGCGTTCGTTCTC
Band lengths:
171-257
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)