DmeEX6001386 @ dm6
Exon Skipping
Gene
FBgn0259734 | CG42388
Description
The gene Nostrin is referred to in FlyBase by the symbol DmelNost (CG42388, FBgn0259734). It is a protein_coding_gene from Dmel. It has 7 annotated transcripts and 7 polypeptides (5 unique). Gene sequence location is X:9030205..9054575. Its molecular function is described by: phospholipid binding; lipid binding. It is involved in the biological process described with: plasma membrane organization; imaginal disc-derived wing hair organization; plasma membrane tubulation; germarium-derived female germ-line cyst encapsulation; regulation of adherens junction organization. 40 alleles are reported. The phenotype of these alleles manifest in: wing hair. The phenotypic classes of alleles include: fertile; flight defective; lethal - all die during embryonic stage; lethal; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, during late pupal stages, in adult female stages.
Coordinates
chrX:9049250-9051410:+
Coord C1 exon
chrX:9049250-9050935
Coord A exon
chrX:9051037-9051122
Coord C2 exon
chrX:9051185-9051410
Length
86 bp
Sequences
Splice sites
3' ss Seq
GTTATTGCTATTCTAAACAGGGA
3' ss Score
6.76
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
Exon sequences
Seq C1 exon
GCTATCAGAGCCTGAATGCCCAGAATCTGTTCGTTTCGATTGACGAACTGCAGGAGCTAACGCGTCAGATCAACGAAACGGAGGATTTTGGCAAGGAGATCGATCTGGAGTACTGCGCGCATCGCGATCAATTGAGACCCAGTGAACGCAGGATCACCCTGCTGAAGAACAAAAACCAAACGTTGATCAACTTCAATCACAACAAGGAGAAGCTGCGTAAGGGCTGGCACGGCATGAAGCACTGGCTGGGCGAGGAGGGCTCCAAGATTAAGGAGGCAGTGCGTCAACAGACGCCCCTCAAACGTCTGGCCCAATCACGGAGCAATCTTAACCAGTCAACTGCGGATGCCAGTCGCCAGTCAATGTCGCCGGAACGCACTCAACGCGATGTAACCGAGAGCTGTGAGGATGTCACCGAACGCACCGAAATGGAGTCGTCCATGTCGCACCGAAACAGCGAGGAGGATCTGTCGCCGCATGCCAAGCGGTTCAAGGATGAG
Seq A exon
GGACAGAACGGCTTCGAGGAACTCCGGCGATATGTGAAGCAGGGCGGTGATTTTAGCAAGGAGCTCATCTTTGTCCTGCAGGAGAG
Seq C2 exon
AGCCGATTCGGAGCTGATCTACTCCAAGTCGCTATCGAAACTGGCCAACAAGCTGAACAAAGCGGGCAGGGAAATTCCCGGGAGCGTGGCCGACGCCTGGCGTGGAGTGGCTACGGAGATGGAGAGCCGCAGCGACATCCATCGCCAGTTGGCAGCCTCGCTAACGGATGAGCTGGTCAAGCCGCTCAAGATCGTCGTCGAAGGACATCACAAGGCGCGCAAGGCG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0259734-'9-9,'9-8,11-9=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.710 A=0.103 C2=0.154
Domain overlap (PFAM):
C1:
PF0061118=FCH=PU(5.6=0.9)
A:
PF0061118=FCH=FE(31.5=100)
C2:
PF0061118=FCH=PD(60.7=71.1)
Main Inclusion Isoform:
FBpp0310014
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0289277, FBpp0289278, FBpp0292036, FBpp0292037, FBpp0292038
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACTCAACGCGATGTAACCGA
R:
GTTGGCCAGTTTCGATAGCGA
Band lengths:
173-259
Functional annotations
There are 2 annotated functions for this event
PMID: 17570224
Exogenously expressed NOSTRINalpha is distributed over the cytosol and associated with subcellular membranes, whereas NOSTRINbeta (skipping of HsaEX0043381) is localized predominantly to the nucleus. Experiments in CHO cells.
PMID: 18980613
This study shows that nuclear import of NOSTRINbeta (skipping of CDS exon HsaEX0043381, inducing the use of a downstream ATG) depends on two nuclear localization signals (aa 32-36: KKRK and aa 57-61: KAKKK). Each of the sequences is independently functional, but both are required to sustain nuclear localization of NOSTRINbeta. Export of NOSTRINbeta from the nucleus is facilitated by a CRM1-dependent mechanism relying on the nuclear export sequence LELEKERIQL (aa 135-145). Unlike NOSTRINbeta, the full-length variant NOSTRINalpha was conspicuously absent from the nucleus. This is most likely because of the fact that its N-terminal F-BAR domain, which is truncated in NOSTRINbeta, facilitates association with cellular membranes. NOSTRINbeta directly binds to the 5'-regulatory region of the NOSTRIN gene (bp -200 to -1), and overexpression of NOSTRINbeta strongly decreases transcription of a reporter gene under control of this DNA region.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)