RnoEX0060444 @ rn6
Exon Skipping
Gene
ENSRNOG00000006611 | Nostrin
Description
nitric oxide synthase trafficking [Source:RGD Symbol;Acc:727920]
Coordinates
chr3:55369214-55389992:+
Coord C1 exon
chr3:55369214-55369356
Coord A exon
chr3:55379324-55379409
Coord C2 exon
chr3:55389909-55389992
Length
86 bp
Sequences
Splice sites
3' ss Seq
CCTGTTATTTTTTCCCCCAGTAC
3' ss Score
9.33
5' ss Seq
AAGGTACGA
5' ss Score
9.78
Exon sequences
Seq C1 exon
ACTGACGTACTCTAAGCCCTTTGAATCCCGGAAGTCCAGAACACACCCAACCTAATTGATTGCAGCGTCCAGCATGCTGGAGCGTAGGTAAAAGAAAAAGCAGACAACATTTCAACATGAGGGACCCACTGACAGACTGTTCG
Seq A exon
TACAATAAAGTATACAAGAGCCTGAAGGAGTTTGCTCAAAATGGAGACAATTTCTGCAAGCAGATCACATCCGTTCTTCAGCAAAG
Seq C2 exon
GGCCAACCTGGAGATCAGCTATGCCAAAGGGCTTCAGAAACTGGCAGTCAAGCTCAGCAAAGCACTACAGAGCACAAAGAAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006611-'1-4,'1-2,2-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061118=FCH=PU(2.3=22.2)
A:
PF0061118=FCH=FE(32.2=100)
C2:
PF0061118=FCH=FE(32.2=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCCGGAAGTCCAGAACACA
R:
TGACTGCCAGTTTCTGAAGCC
Band lengths:
169-255
Functional annotations
There are 2 annotated functions for this event
PMID: 17570224
Exogenously expressed NOSTRINalpha is distributed over the cytosol and associated with subcellular membranes, whereas NOSTRINbeta (skipping of HsaEX0043381) is localized predominantly to the nucleus. Experiments in CHO cells.
PMID: 18980613
This study shows that nuclear import of NOSTRINbeta (skipping of CDS exon HsaEX0043381, inducing the use of a downstream ATG) depends on two nuclear localization signals (aa 32-36: KKRK and aa 57-61: KAKKK). Each of the sequences is independently functional, but both are required to sustain nuclear localization of NOSTRINbeta. Export of NOSTRINbeta from the nucleus is facilitated by a CRM1-dependent mechanism relying on the nuclear export sequence LELEKERIQL (aa 135-145). Unlike NOSTRINbeta, the full-length variant NOSTRINalpha was conspicuously absent from the nucleus. This is most likely because of the fact that its N-terminal F-BAR domain, which is truncated in NOSTRINbeta, facilitates association with cellular membranes. NOSTRINbeta directly binds to the 5'-regulatory region of the NOSTRIN gene (bp -200 to -1), and overexpression of NOSTRINbeta strongly decreases transcription of a reporter gene under control of this DNA region.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]