DreEX0007303 @ danRer10
Exon Skipping
Gene
ENSDARG00000075924 | RAPGEF4 (1 of 2)
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr6:6755285-6759330:+
Coord C1 exon
chr6:6755285-6755410
Coord A exon
chr6:6755839-6756017
Coord C2 exon
chr6:6759243-6759330
Length
179 bp
Sequences
Splice sites
3' ss Seq
TTGCGTGTGTTTGTGTGCAGTAT
3' ss Score
7.34
5' ss Seq
CCAGTGAGT
5' ss Score
8.28
Exon sequences
Seq C1 exon
AATCCACTTCCAGAGCAGGAGGGTCCGTCCACAGGTTCTATGTCCAGTTTTGAGCTGATGAGCTCTAAAGATCTGGCCTTTCAGATGACCCAGTACGACTGGGAACTTTTCAGCTGCGTACATGAG
Seq A exon
TATGAGCTGGTGTATCACACATTTGGACGGCAGGCGTACCGACGATCCACAGCCAATCTGGAGCTGTTTCTGCGGCGCTTCAATCAGCTGCAGCTGTGGGTGGTGACGGAGGTCTGTCTCTGCGGGACGCTCAGCAAACGGGTGCAGCTCTTGAAGAAGTTCATCAAGATCGCCGCCCA
Seq C2 exon
GATCCATCTAGGAATCATCGTGCTTACCGCCTCACTGTGGCCAAGATGGAGCCGCCAATCATTCCCTTTATGCCCCTCCTCATTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075924_MULTIEX1-12/17=11-15
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.095 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0078818=RA=PD(20.6=52.4),PF0061714=RasGEF=PU(13.0=57.1)
A:
PF0061714=RasGEF=FE(31.9=100)
C2:
PF0061714=RasGEF=FE(15.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCACTTCCAGAGCAGGAGG
R:
TAATGAGGAGGGGCATAAAGGGA
Band lengths:
210-389
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]