DreEX0018196 @ danRer10
Exon Skipping
Gene
ENSDARG00000026855 | cacna2d4a
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4a [Source:ZFIN;Acc:ZDB-GENE-041210-213]
Coordinates
chr4:20559359-20569202:-
Coord C1 exon
chr4:20569071-20569202
Coord A exon
chr4:20568729-20568789
Coord C2 exon
chr4:20559359-20559509
Length
61 bp
Sequences
Splice sites
3' ss Seq
TTTTATATATTTTTTCTAAGGTA
3' ss Score
8.57
5' ss Seq
CTGGTAAGT
5' ss Score
10.65
Exon sequences
Seq C1 exon
ATCCTGATATCCTGAACGGGGTGTTTATGTCAGAAGCACTGAATGACATATTTATCAGTAACTTTGAGAAGGACCCGACTCTAACCTGGCAGTACTTCGGCAGTTCAACTGGATTCTTTAGACTTTACCCAG
Seq A exon
GTATTAAGTGGACACCTGATGAAAATGGAGTTGTCACTTTTGATGGAAGGAATCGCAACTG
Seq C2 exon
GTACATTCAAGCAGCCACATCTCCTAAAGACATCATTATCGCTGTGGACATCAGCGGGAGTATGAAGGGTTTGAGACTCACCATCGCAAAACACACCATCAACACCATTCTAGACACACTAGGCGAAAACGACTTTGTCAACGTCATTGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000026855-'6-6,'6-4,7-6
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF083996=VWA_N=FE(37.6=100)
A:
PF083996=VWA_N=PD(3.4=19.0)
C2:
PF137681=VWA_3=PU(24.7=80.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCAGTACTTCGGCAGTTCAA
R:
TGGTGTGTTTTGCGATGGTGA
Band lengths:
143-204
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]