DreEX0018207 @ danRer10
Exon Skipping
Gene
ENSDARG00000023886 | cacna2d4b
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4b [Source:ZFIN;Acc:ZDB-GENE-100422-18]
Coordinates
chr25:18979278-18992528:-
Coord C1 exon
chr25:18991776-18992528
Coord A exon
chr25:18983102-18983233
Coord C2 exon
chr25:18979278-18979338
Length
132 bp
Sequences
Splice sites
3' ss Seq
TCAGTCATGTTTATTTGCAGATC
3' ss Score
8.98
5' ss Seq
CAGGTTTAA
5' ss Score
1.88
Exon sequences
Seq C1 exon
AGATGTCATTCTTTGAGGATGAGATGATGAAGTTCAACTTAAAACATTGATATCATGATTTCGACCAGTAGGAAGAGCAAGCTAACGATGACATTAAGTGGATTATTTGGATTATCTGTATCATCCTGAATATGAGAAGTGTTTGTTACTTCAGGCAGCTCACTAGGACTTTGAGAAGTTTCTGGAATATCTGAGGATGACAGGAATCAACTTCTGGATTGATGAGGAGTTCAGAAGGGTCCTAAGAGACGTTTAAGACCATCTAGGTCAATATGAATGGAAGCCATTGGATTGAAAATTGGTGTTTATTTAAGACACTGGAGTAGTGGATTCTTAGACTTCTTCTCATGACTTCATTATACTTTTGTGGCCTTTAAGGATCCAGTAGGTGCCAGCATCATGTGTCACATCAGAAGGTCTTCCTGGGTCACATTTCTGTTGCTCTCCACAGGCTGGATGTGCATGTTTACTCATGCCCAAATGAAGATTCCACCTGAAAC
Seq A exon
ATCCAGATATTCTCAATGGAGTGTACATGTCAGAGGCCTTGAACGACATCTTCATCAATAACTTCCAGAAAGATCCCACGCTCACCTGGCAGTATTTCTGCAGCCAAACGGGTTTCTTCAGACTTTATCCAG
Seq C2 exon
GAATCCGATGGACCCCTGATGCTAACGGTGTTGTGTCTTTTGACTGCAGAAACAGAAACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000023886_MULTIEX1-4/4=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF083996=VWA_N=FE(37.6=100)
C2:
PF083996=VWA_N=PD(2.6=14.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCCATTGGATTGAAAATTGGTGT
R:
CAACACCGTTAGCATCAGGGG
Band lengths:
252-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]