DreEX0021698 @ danRer10
Exon Skipping
Gene
ENSDARG00000003420 | chrna5
Description
cholinergic receptor, nicotinic, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-050417-440]
Coordinates
chr18:40499317-40502277:-
Coord C1 exon
chr18:40502177-40502277
Coord A exon
chr18:40501926-40502014
Coord C2 exon
chr18:40499317-40499667
Length
89 bp
Sequences
Splice sites
3' ss Seq
TTATCTTCCGTCTAATGGAGGGG
3' ss Score
-5.82
5' ss Seq
AAGGTGACT
5' ss Score
6.64
Exon sequences
Seq C1 exon
CAATCCTCTTCTTTTAAAGGTCGACATTCTCCTTGAAGACGTGCATGTGGACAAACGTGACTATTTTGACAATGGCGAATGGGAGATCGTGAAGGCCACTG
Seq A exon
GGGAAAAAATCTCCCTCTGCACGTCTGTCCTCGTCTCACTTACTGTCTTCCTTCTGGTGATAGAAGAGATCATCCCTTCCTCCTCAAAG
Seq C2 exon
GTAATTCCTCTGATTGGAGAATATTTGGTCTTCACTATGATCTTCGTGACACTTTCCATTGTCATCACCGTCTTTGCTATTAACATCCACCATCGCTCCTCTTCCACCCATCATAGCATGGCTCCCTGGGTCCGCCGTATCTTTCTTCACCACTTGCCTAGACTGCTCTGCATGCGTAGCCACGTGGACCGTTACGCCACCAGCGCTGGGACTCGAACAGGAGGACTGGGCTATGGGAAGAGTTTGAGTCAGGATACCAACCAGCTCGATGCAGAACACAACCTGCAGGCGGCCTTAGAGTCTATACGCTACATCACACGCCATGTGGTGAAGGAGAATGAAGTCAGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000003420-'9-12,'9-11,10-12
Average complexity
C1*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NA
A:
PF0293118=Neur_chan_LBD=PD(25.5=46.8),PF0293211=Neur_chan_memb=PU(40.8=45.9)
C2:
PF0293211=Neur_chan_memb=PD(57.6=61.5),PF0293211=Neur_chan_memb=PU(60.7=43.6)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGAATGGGAGATCGTGAAGGC
R:
GAAGAAAGATACGGCGGACCC
Band lengths:
174-263
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]