BtaEX6077430 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000013657 | CHRNA5
Description
Bos taurus cholinergic receptor, nicotinic, alpha 5 (CHRNA5), mRNA. [Source:RefSeq mRNA;Acc:NM_174514]
Coordinates
chr21:31489738-31495957:+
Coord C1 exon
chr21:31489738-31489847
Coord A exon
chr21:31491762-31492593
Coord C2 exon
chr21:31495754-31495957
Length
832 bp
Sequences
Splice sites
3' ss Seq
TGTGTGTGTGTGTATTTCAGTGC
3' ss Score
10.75
5' ss Seq
GAGGTCTGT
5' ss Score
5.39
Exon sequences
Seq C1 exon
GAGTGGATAGATGTGAAGTTGAGATGGAACCCTGATGACTACGGTGGAATAAAACTTATACGTGTCCCTTCAGACTCTCTCTGGACTCCAGATATCGTTTTGTTTGATAA
Seq A exon
TGCAGATGGACGTTTTGAAGGGGCCAGTACCAAAACCGTCGTCAGGTACGACGGCACGGTGACCTGGACTCCACCGGCAAACTACAAAAGTTCATGTACCATCGACGTCACATTTTTCCCATTTGATCTCCAAAACTGTTCCATGAAATTTGGTTCTTGGACTTACGACGGATCACAGGTTGATATAATTTTGGAGGACCAAGATGTAGACAAGAGAGATTTTTTTGATAATGGAGAATGGGAAATTGTCAGCGCAACAGGGAGCAAAGGAAACAGAACCGACAGCTGTTGCTGGTATCCTTACATCACTTACTCGTTTGTAATTAAGCGTCTGCCTCTCTTTTATACCTTGTTCCTCATTATACCCTGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAATGAAGGTGAAAAGATCTGTCTCTGCACTTCAGTACTTGTGTCTCTGACTGTCTTCCTTCTCGTTATTGAAGAGATCATACCCTCATCTTCCAAAGTCATACCTCTGATTGGAGAGTACCTGGTGTTCACGATGATTTTTGTGACACTGTCTATCATGGTCACTGTCTTTGCTATCAACATTCATCACCGTTCTTCCTCAACGCACGACGCTATGGCTCCGTGGGTCCGCAAGATATTTCTTCACAAGCTACCGAAACTGCTTTGCATGCGAAGTCATGTAGACAGGTACTTCAGTCAGAAGGAGGAAGCTCGGAGCAGCCGTGGACCCAGATCTTCTAGAAACGCCCTGGAAGCTGCACTGGATTCCGTTCGCTATATCACAAGACACGTCATGAAGGAGACCGATGTCCGTGAG
Seq C2 exon
GTTGTTGAAGATTGGAAATTCATAGCCCAGGTTCTTGATCGGATGTTTCTGTGGACTTTTCTTCTGGTTTCAGTTGTTGGATCTCTTGGGCTTTTTGTTCCTGTTATCTATAAATGGGCAAATATAATAGTACCAATTCATATTGGAAATGAAAATAAGTGACACTTTCCAAGGGGTTGTAGTAGGAATTTAGTTAAAAGGCAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000013657-'4-12,'4-4,5-12=AN
Average complexity
A_S
Mappability confidence:
90%=100=83%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.011 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(17.6=100)
A:
PF0293118=Neur_chan_LBD=PD(54.9=40.3),PF0293211=Neur_chan_memb=WD(100=47.8),PF0293211=Neur_chan_memb=PU(57.7=16.2)
C2:
PF0293211=Neur_chan_memb=PD(39.7=57.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGGATAGATGTGAAGTTGAGATGG
R:
ACTAAATTCCTACTACAACCCCTTGG
Band lengths:
303-1135
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]