DreEX0021704 @ danRer10
Exon Skipping
Gene
ENSDARG00000069660 | chrnb1
Description
cholinergic receptor, nicotinic, beta 1 (muscle) [Source:ZFIN;Acc:ZDB-GENE-070821-4]
Coordinates
chr7:22278777-22283289:+
Coord C1 exon
chr7:22278777-22278910
Coord A exon
chr7:22282923-22282967
Coord C2 exon
chr7:22283180-22283289
Length
45 bp
Sequences
Splice sites
3' ss Seq
TTTTGTGTTTTGTGCTTTAGAAC
3' ss Score
10.28
5' ss Seq
TTGGTAAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
GTGCAACCGAAATGGAAGATAAGCTGATGAAGAAACTGTTTTCTGCATACAACAGTAAAGTTCGGCCTGCAAGGAACCCAGAAGAAAGGGTTGTTGTGAGGATAGGCATGATACTTTCATCGTTCGTCAGCCTG
Seq A exon
AACATGAAAGATGAGGAGATGAACACCATTGTTATGATGAACTTG
Seq C2 exon
GAGTGGAATGATTATCGCCTCTCCTGGAATCCCAAAGATTATGGAGGAGTTGATGTATTGCGGATCTCTTCAGGAAAAGTGTGGCTTCCTGATATTGTTCTCATCAACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000069660-'2-3,'2-2,3-3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=PU(18.6=86.7)
A:
PF0293118=Neur_chan_LBD=FE(6.7=100)
C2:
PF0293118=Neur_chan_LBD=FE(17.1=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTAAAGTTCGGCCTGCAAGG
R:
CCGCAATACATCAACTCCTCCA
Band lengths:
144-189
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]