DreEX0021715 @ danRer10
Exon Skipping
Gene
ENSDARG00000038508 | chrnb3b
Description
cholinergic receptor, nicotinic, beta 3b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-030102-4]
Coordinates
chr14:41158120-41162886:+
Coord C1 exon
chr14:41158120-41158229
Coord A exon
chr14:41159692-41160568
Coord C2 exon
chr14:41161808-41162886
Length
877 bp
Sequences
Splice sites
3' ss Seq
TATCACTTGCCTGCTTGTAGCGC
3' ss Score
7.31
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
Exon sequences
Seq C1 exon
GAATGGCTGGATTATAAACTGCGCTGGAACCCTGAGAATTATGGTGGGATCACATTTATCAGAGTCCCGTCAGAGAGCATCTGGCTCCCAGACATCGTTTTATATGAAAA
Seq A exon
CGCAGACGGACGTTTTGAAGGCTCGATCATGACCAAAGCCATCGTGAGATACAATGGCCTGATCACCTGGACTCCTCCCGCCAGCTACAAATCTGCCTGCACCATGGACGTGACCTTCTTCCCGTTCGATCGGCAGAACTGCTCCATGAAGTTCGGCTCCTGGACCTACGATGGAAACATGGTGGAGCTAGTCCTTGTTAACCATCAGGTGGACCGAAGCGACTTCTTTGACAATGGCGAATGGGAGATTCTCAGCGCGACAGGGCACAAAGGCAGCCGACGGGACGGTCATTACTCCTACCCCTACATCACATACTCATTCATCCTAAAGCGCCTTCCTCTTTTCTACACGCTCTTTCTTATCATCCCGTGTCTGGGTTTGTCTTTCCTGACCGTTCTGGTTTTCTACCTTCCCTCGGACGAAGGAGAAAAAGTCTCTCTATCCACCTCCGTCCTGGTGTCGCTCACCGTCTTCCTTCTCGTCATCGAAGAAATCATTCCGTCTTCCTCTAAAGTGATCCCTCTGATCGGAGAGTATCTGCTTTTCATCATGATCTTCGTGACACTCTCCATCATCGTGACGGTGTTTGTTATTAACGTTCACCATCGCTCTTCAGCCACGTATCATCCCATGTCTCCGTGGGTTCGTACTCTGTTCCTCCAGCGGCTGCCGGATTTGCTGTGTATGAGGGGAAACACCGACCGCTACCATTACCCGCAGATGGAGCCCCAGAGCCCTGATCTGAAGCCCAGGAGCAAGAAAGGGCCACCTGGGCCTGAAGGAGACGGTCAGGCGCTGATCAACATGCTGGAGCAGGCCACTAACTCTGTGCGCTACATCTCACGGCATATCAAGAAGGAGCATTTTATTAGAGAG
Seq C2 exon
GTTGTACAGGACTGGAAGTTTGTAGCTCAGGTTCTGGACAGGATTTTTTTGTGGGCTTTCCTCACCGTCTCTGTGCTCGGCACCATCCTGATCTTCACTCCTGCGGTGAAGATGTTTCTGAGCACTCCTCCACCTCCGTCTCCTTAACACTCATCCACCAAAATCAGCAATCAAGACCTGAATACTGTACTTACAGTAACATACACACAGCACTCAGTATTAGGTAGACCTTCTTTCACTACTTTGAGATATATATAAAATGTATTATTATTATTATTATTATTATTATTTGTTGATTGTTAGGTCTGTCTTTTTTACACATATTTCCTAAACAACTTTTAGCAATTACTTGCACATACAAAATGCAGTAAAAGTAACAGTGTACAAATCATTCCAGAATTGCGGGTACATTGAAAACATTTTGCATTTATTTTTCTTGACTGGAATGACATAAAGCATACAAATAATGAACAGTAAATGTAATACAATAGCTAATTGTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000038508-'5-8,'5-5,7-8
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.096 C2=0.125
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(17.6=100)
A:
PF0293118=Neur_chan_LBD=PD(55.1=38.6),PF0293211=Neur_chan_memb=WD(100=49.5),PF0293211=Neur_chan_memb=PU(61.2=17.7)
C2:
PF0293211=Neur_chan_memb=PD(36.5=63.3)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATAAACTGCGCTGGAACCCTG
R:
TCTCAAAGTAGTGAAAGAAGGTCT
Band lengths:
346-1223
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]