MmuEX6035190 @ mm9
Exon Skipping
Gene
ENSMUSG00000031492 | Chrnb3
Description
cholinergic receptor, nicotinic, beta polypeptide 3 [Source:MGI Symbol;Acc:MGI:106212]
Coordinates
chr8:28503772-28510201:+
Coord C1 exon
chr8:28503772-28503881
Coord A exon
chr8:28504086-28504968
Coord C2 exon
chr8:28507132-28510201
Length
883 bp
Sequences
Splice sites
3' ss Seq
GGTAACCACTTTCATGGCAGTGC
3' ss Score
3.21
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
Exon sequences
Seq C1 exon
GAATGGACAGACCAAAAATTACGATGGAATCCCGAAGACTATGGTGGAATTAATTCGATAAAGGTTCCATCGGAGTCGCTCTGGCTGCCTGACATAGTTCTCTTTGAAAA
Seq A exon
TGCTGACGGACGTTTTGAGGGCTCTCTCATGACCAAGGCCATTGTGAAATCCAGCGGAACCGTCAGTTGGACTCCTCCCGCCAGCTACAAAAGTTCCTGTACCATGGATGTCACATTTTTCCCGTTCGATAAGCAGAACTGCTCAATGAAGTTCGGATCCTGGACTTACGATGGTACCATGGTCGACCTCATTCTGATCAATGAAAACGTTGACCGGAAAGACTTTTTTGATAACGGAGAGTGGGAGATACTAAATGCAAAGGGGATGAAGGGCAACAGAAGAGAAGGCTTTTACTCCTATCCGTTTGTTACCTACTCTTTTGTTCTGAGACGCCTGCCCCTGTTTTACACCCTCTTTTTGATAATCCCCTGCCTGGGGCTGTCTTTTCTCACGGTCCTGGTGTTCTACCTACCCTCCGACGAAGGGGAAAAACTCTCCTTATCCACCTCGGTTTTGGTTTCTTTGACAGTGTTTCTTTTAGTAATCGAAGAAATAATCCCGTCTTCTTCAAAAGTCATCCCTCTCATTGGGGAGTACCTCCTCTTCATTATGATATTTGTCACGCTGTCTATTATTGTCACGGTTTTTGTAATTAATGTCCACCACAGATCTTCCTCAACGTACCATCCCATGGCCCCCTGGGTGAAGAGGCTGTTTCTGGAAAAACTCCCGAGATGGCTTTGCATGAAGGACCCCAGGGACCGCTTCTCTTTCCCCGATGGAACGGAGAGTAAGGGAACCGTGAGGGGGAAATTTCCAGGGAAAAAGAAGCAGACTCCTACCAGTGATGGAGAAAGAGTTCTGGTCGCTTTCCTGGAGAAGGCCTCTGAGTCCATCAGATACATCTCGAGGCATGTGAAGAAGGAACACTTCATCAGTCAG
Seq C2 exon
GTTGTTCAAGACTGGAAATTCGTGGCTCAGGTTCTTGACCGCATCTTCCTCTGGCTCTTTCTGACAGCTTCGGTTTTGGGCTCTGTCCTGATTTTTATTCCAGCCTTGAAGATGTGGATACATCGTTTCCATTAGGAACTGCTCTCTGGATCCATGTAGAAGACACACATAGAGAGAATCCCACCTAGAACTGAGGGCTGCTGGTGTGTTCACAGAAAGGAGTTGCATGCAACAGTTGTGGTGCCCTTGCCTTGTCCTGGGAGCCTTCTGTGACTGGAGGAAACTGAGAAAACGTGGGTTTGAGCTAGTGTGATGGTGGCTGCCATTAGAGATGTATTTGGGCAATTCAGAGAAGTCTCCATGTCATGTTGTTGTGTGGGAGGCTCCGAAATGCTCCATTTGCCTATCCCTGAACTACTGCATCTGCCCATCCCCCAGACTTCTACATCTGCTCATCCCTAAACTCTTGATTTTGTCCATCCACTGAACTCCTAGTATCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031492-'3-4,'3-3,4-4=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.031 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(17.6=100)
A:
PF0293118=Neur_chan_LBD=PD(55.1=38.3),PF0293211=Neur_chan_memb=WD(100=43.7),PF0293211=Neur_chan_memb=PU(62.1=18.3)
C2:
PF0293211=Neur_chan_memb=PD(35.6=68.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCTGCCTGACATAGTTCTCT
R:
AGCCACCATCACACTAGCTCA
Band lengths:
350-1233
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: