DreEX0031878 @ danRer10
Exon Skipping
Gene
ENSDARG00000016156 | fam53b
Description
family with sequence similarity 53, member B [Source:ZFIN;Acc:ZDB-GENE-040426-2495]
Coordinates
chr12:46903628-46921771:+
Coord C1 exon
chr12:46903628-46903682
Coord A exon
chr12:46914202-46914860
Coord C2 exon
chr12:46921056-46921771
Length
659 bp
Sequences
Splice sites
3' ss Seq
CTCTCTCCGCTGTGTTGCAGACT
3' ss Score
9.29
5' ss Seq
CAGGTAAAC
5' ss Score
7.82
Exon sequences
Seq C1 exon
CAGGAGCCGCTGACCATGAGCCAGGGGACAGCTCTGTTCTCCTGTGGAATCATGG
Seq A exon
ACTCTGGGCGCTGGGCAGATGTTGGGAGTGTGTGTGGCGTCCAGCAGCGGCCTGTGGGCTCCAGTCTGGAGAGCCTGTGGGACAGCATGCGTGAGGCGGGCGCCACTGGCGGCATCAGCGGGTTACTGCGGGATCTGAGTCTGAGCGAAGCGTCTCCTGCCAGTGCTGCGCCGCCCAGCAAACGCCAGTGCCGCTCGCTGTCCTGCTCGGATGAGCTGGGCTGCCGCTCATCCTGGCGTCCGCAGGGTTCGCGCGTCTGGACAACGGTGGAAAAGCGGCGCTGCCACAGTGGAGGGAGCGTCCAGAGAGGAGTCTTCAATCCATCAGGCTTTCCTGCAATGCAGCGCAGCTCCAGCTTCAGTCTGCCGGCGCACAGCAGTCATCTGGAGCCCTTCACTCACGGTTTCCCATTCCAGGCATTCTCCGAGTGTCCGCAGACGCCGCAGACGCTGTACCGTTCACATGAGCAGATCTGCCCGGCGGAGGCCAGCTCCCCTGAATCCACCCCAGAGCTCCAGCGCCGCAGCGGGCAGAGCGGACTCGCACGCAGCCGATCACAGCCCTGCGTACACAACCATCAGAAGATCGGCGTCAAGCGCAGGAGACCGGCCGACTCGCACAAACAGAGGCCCTCGCTGGACCTGCTCAAGATGACACAG
Seq C2 exon
AAGCTGCAGGATTTTCACAGTCTCAGCTGTCCAGGATTCAGCGGCGACGACAAAACTCTGCCGTCCAGCAGCCCGGCACTCCTCAACGACACATGTGAGCGTGCTCAGAATGACAGCTCAGCGGATGCCCCCATCCACCAATCAGAGAGCAGCTCAGAGGACGCCCTCATCCACCAATCAGATAGCAGCTCAGCGGACGCCCTCATCCACCAATCAGAAAGCAGCAGGCCCGCAGGAAAGGAGCGCGAATGCTTGTGGGCGGGGCTTTGTAGCAGAAGGGGCCGGGACTTATTCCAGCTGGGCGGAGAACTGGATATCGAGCAGATTGAGAGGAACTGAGAAGCATTTCTTGTTGGATTATTATGGGATGTACGCTTCAGTAAGTGATGCAGGACACATGTAATCGACTCAGGGTGAAGCACATGGAGGACGTGATCCGAGCTCCGTGTGGCGCAAGAGAACACGCACACATTTAGATTAGCAGAGCCATTTGACATTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000016156_MULTIEX1-3/3=2-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.211 A=0.381 C2=0.652
Domain overlap (PFAM):
C1:
PF152421=FAM53=FE(9.2=100)
A:
PF152421=FAM53=PD(77.6=69.1),PF152421=FAM53=WD(100=37.3)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGCTCTGTTCTCCTGTGGA
R:
GCTCGATATCCAGTTCTCCGC
Band lengths:
350-1009
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]