DreEX0038449 @ danRer10
Exon Skipping
Gene
ENSDARG00000089212 | htr3a
Description
5-hydroxytryptamine (serotonin) receptor 3A [Source:ZFIN;Acc:ZDB-GENE-071012-5]
Coordinates
chr21:23098790-23099670:+
Coord C1 exon
chr21:23098790-23098940
Coord A exon
chr21:23099375-23099538
Coord C2 exon
chr21:23099626-23099670
Length
164 bp
Sequences
Splice sites
3' ss Seq
AATGGTTTATCTTGGTGTAGTGA
3' ss Score
3.88
5' ss Seq
GTGGTGGGT
5' ss Score
4.29
Exon sequences
Seq C1 exon
AATGGAGTTCAGCCTGCATCACTAGATGGAAGCAGCACCAGGCTAGAAGAACAGTTATAGAGCAACTGCGAATCGCTCAGCACAATGAGAAGTTCAGCACTCTGGACAGCTCTCTGCATATGCATGTATGGCACAGTGGTGGATTGCTCAG
Seq A exon
TGAAGAAGCTTGGCAATAACACTGGGCGTTTCGCCAATGCCACTCTGGTTCGGCTAAATGAGTTTTTAAGTGCCGGATATAAAAAAGGAGTTCGACCTGTGCGCGACTGGAGGCAGTCAACGACGGTGGCAATTGACCTTATGGTTTATGCCATTCTCAATGTG
Seq C2 exon
GATGAGAAGAACCAGGTTTTGACAACATATGTGTGGTACAGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000089212_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0146213=LRRNT=PU(71.4=65.2)
A:
PF0146213=LRRNT=PD(23.8=9.1),PF0293118=Neur_chan_LBD=PU(18.7=70.9)
C2:
PF0293118=Neur_chan_LBD=FE(6.7=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGGAGTTCAGCCTGCATCAC
R:
TGTCTGTACCACACATATGTTGTCA
Band lengths:
194-358
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]