DreEX0039204 @ danRer10
Exon Skipping
Gene
ENSDARG00000105177 | ilf3b
Description
interleukin enhancer binding factor 3b [Source:ZFIN;Acc:ZDB-GENE-030131-587]
Coordinates
chr3:13716566-13727858:+
Coord C1 exon
chr3:13716566-13716610
Coord A exon
chr3:13723889-13724260
Coord C2 exon
chr3:13727074-13727858
Length
372 bp
Sequences
Splice sites
3' ss Seq
CATGTACTATTCCTCTTCAGATT
3' ss Score
9.54
5' ss Seq
AAGGTGAAC
5' ss Score
5.54
Exon sequences
Seq C1 exon
GTGGATATGGGACGTATGGATATGGTGGCAATAACTCTGGCTACA
Seq A exon
ATTTCTATAATAATGGTGGCTCTAATGGCGGATCTGTGGCCTCAAATCAAGGCACAAACCAGTCTTCAGGTGGAGCGGCTGCGACTGGCGGATTCGGCTCATACTATCAGAGCGACAACAGCTACACTTCCCCTGCCCCAACCCCTAAACCTGTAGGCAAAAAGCCACCCATGCACCAAAACACCAAGCCTCAGGCCCCCGGAGTGGGCCAGGGTGGCTCCTACGGTCAGTACAATCAAGGCTACGGGCAGAAGAAGAACTTCGGTCAGGCTCAAGGTGGTGGTGGAGCAGCTGCTGGTGGTGGAGGAAACTTCAATTACAGCACTGCTTATCCCTCACAGGTGACCGGAGGACAGGAGTACAACTATGAAG
Seq C2 exon
GTTACAGCAACCAGTCCAACTACAACTCCCAGGGTGGAGCCAACCAGAATTTCGGCGGTAACTCTGCCCCGTACAACAGCGGCCAAGCCGGGTACGGCCGGGGCGATCCCAATATGAACTACCAGTACCGATAAACCTGCCTTCCCACGCGCAGTCCTGAAGCATCATTTCCAAACAAGGAGCATCTTCCTCTTTGATTCTGTTGTTCTTGAGTTTTGGTTTCTGTCGCCAACATCTAAACTAAAGGAAAATAGCAGTCTCTAAACTTTGTGCACAAATAGTGTATCTGCGTATTATTTTGAAGCTTGTTGTATTCTTAATTTGAGTTATTTCATATCTGTGAACGCCCCCCTGAACTACTGCCCTCTAGAGGGACACGAGAACTACATCCCAAAGCGACGCATGTGAGGACCTGATGTTAATATGCAGTGACGGGACACTCTGTCCTTGAGTGATTTTTAACCATTTAAACCTGTATAAATATAAGACGCACCAGTCTC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000105177-'23-35,'23-34,27-35
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.128 A=0.972 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGACGTATGGATATGGTGGC
R:
ACAACAAGCTTCAAAATAATACGCA
Band lengths:
349-721
Functional annotations
There are 1 annotated functions for this event
PMID: 30796096
ILF3 isoform-1 (FL) and isoform-2 (APA creating exon 18) promote cell proliferation and transformation. Tumor cells with reduced SRSF3 expression produce aberrant isoform-5 (APA and Alt3) and -7 (skipping of exon 19) of ILF3. ILF3 isoform-5 and isoform-7 suppress tumor cell proliferation and the isoform-7 induces cell apoptosis.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]