RnoEX0043976 @ rn6
Exon Skipping
Gene
ENSRNOG00000022741 | Ilf3
Description
interleukin enhancer binding factor 3 [Source:RGD Symbol;Acc:619734]
Coordinates
chr8:22435560-22440410:+
Coord C1 exon
chr8:22435560-22435613
Coord A exon
chr8:22438742-22439107
Coord C2 exon
chr8:22440274-22440410
Length
366 bp
Sequences
Splice sites
3' ss Seq
TCCGCTGCTTCCCTGTCTAGGTC
3' ss Score
11.86
5' ss Seq
TCAGTGAGT
5' ss Score
7.68
Exon sequences
Seq C1 exon
GCGCTGGATATGGAAGCTATGGGTACAGCAGCAATTCGGCCACAGCAGGCTACA
Seq A exon
GTCAGTTCTACAGCAATGGAGGGCATTCTGGGAATGCCGGTGGTGGAGGCAGCGGGGGAGGTGGTGGCTCGTCCAGCTACAGCTCCTACTACCAAGGAGACAGCTACAACTCACCAGTGCCCCCGAAACATGCTGGGAAGAAGCCGCTGCATGGGGGCCAGCAGAAACCCTCCTACAGCTCGGGCTACCAGTCCCACCAGGGCCAGCAGCAACCTTACAACCAGAGCCAGTACAGCAGCTACGGCACGCCACAGGGCAAGCAGAAAGGCTATGGCCATGGGCAGGGCAGCTACTCCTCCTACTCCAACTCTTACAACTCCCCTGGTGGTGGTGGGGGCTCTGACTACAGCTACGACAGCAAATTCA
Seq C2 exon
GAGGCTACTCATCACAGTCAAACTACAGCTCCCCTGGGTCCAGCCAGAGCTACAGCGGCCCCGCCAGCTCCTACCAGTCCTCACAGGGTGGCTACAGTCGGAACACAGAACACAGCATGAACTACCAGTACAGATAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000022741_MULTIEX2-2/3=C1-C2
Average complexity
C2*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.084 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
ENSRNOT00000079230fB6870
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGCTGGATATGGAAGCTATGG
R:
TATCTGTACTGGTAGTTCATGCTGTG
Band lengths:
190-556
Functional annotations
There are 1 annotated functions for this event
PMID: 30796096
ILF3 isoform-1 (FL) and isoform-2 (APA creating exon 18) promote cell proliferation and transformation. Tumor cells with reduced SRSF3 expression produce aberrant isoform-5 (APA and Alt3) and -7 (skipping of exon 19) of ILF3. ILF3 isoform-5 and isoform-7 suppress tumor cell proliferation and the isoform-7 induces cell apoptosis.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]