RnoEX0043971 @ rn6
Exon Skipping
Gene
ENSRNOG00000022741 | Ilf3
Description
interleukin enhancer binding factor 3 [Source:RGD Symbol;Acc:619734]
Coordinates
chr8:22435560-22438549:+
Coord C1 exon
chr8:22435560-22435613
Coord A exon
chr8:22436054-22436114
Coord C2 exon
chr8:22438219-22438549
Length
61 bp
Sequences
Splice sites
3' ss Seq
TCTGTCTCCCCCTTTTGTAGGTG
3' ss Score
11.91
5' ss Seq
AAAGTATTG
5' ss Score
-1.65
Exon sequences
Seq C1 exon
GCGCTGGATATGGAAGCTATGGGTACAGCAGCAATTCGGCCACAGCAGGCTACA
Seq A exon
GTGACTTTTTCACAGACTGCTACGGCTATCATGATTTTGGGGCTTCCTAGAGCGTCTAAAA
Seq C2 exon
GAAGAAAGGAAGCTTGGCAAGTGTCTGAGTCTCAAATCACTGTCTTAAGAGCACTTGGTCTTTGAAGAACCGTGGTGGCTATGGCGAGGCATCTCCTCCTAGGGTCCCCAAGACTTAGGCCTGTTGAGTTCATTTCCTGCACGGAGGGAAAAGGGGGTGGCTCACTGCTTCTGGGTGCTTTGCTTTTCCTGTCACCGTTGGGGACATCTGGGTAGCCGTCAGCTAGTCCTGAATGTCATCCACGCTCGGAGCCCATCTCATTGTAATGTTGACATCTGCAAAGCTGCTGTCGTGCTGCTGCTTGGCAAAGACCCCTTGTCATCAGCTGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000022741-'31-38,'31-37,32-38
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.084 A=0.000 C2=NA
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NA
Main Inclusion Isoform:
ENSRNOT00000079230fB6870
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGGGTACAGCAGCAATTCGG
R:
GGACCCTAGGAGGAGATGCC
Band lengths:
143-204
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa Enriched at 14 days (FC=1.278, FDR=0.002), PC9 No Change at 14 days (FC=0.979, FDR=0.749), Late Xenograft No change (FC=0.994, FDR=0.324).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]