MmuEX0024058 @ mm10
Exon Skipping
Gene
ENSMUSG00000032178 | Ilf3
Description
interleukin enhancer binding factor 3 [Source:MGI Symbol;Acc:MGI:1339973]
Coordinates
chr9:21399960-21403501:+
Coord C1 exon
chr9:21399960-21400013
Coord A exon
chr9:21400452-21400512
Coord C2 exon
chr9:21403136-21403501
Length
61 bp
Sequences
Splice sites
3' ss Seq
TCTGTCTCCCCCTTTTGTAGGTG
3' ss Score
11.91
5' ss Seq
AAAGTATTG
5' ss Score
-1.65
Exon sequences
Seq C1 exon
GTGCTGGATATGGAAGCTATGGGTACAGCAGCAATTCGGCCACAGCAGGCTACA
Seq A exon
GTGACTTTTTCACAGACTGCTACGGCTATCATGATTTTGGGGCTTCCTAGAGCGTCTAAAA
Seq C2 exon
GTCAGTTCTACAGCAATGGAGGGCATTCTGGGAATGCCGGTGGTGGAGGCAGCGGGGGAGGTGGTGGCTCATCCAGCTACAGCTCCTACTACCAAGGAGACAGCTACAACTCACCAGTACCCCCGAAGCATGCTGGGAAGAAGCCGCTGCATGGGGGCCAGCAGAAAGCCTCCTACAGCTCGGGCTACCAGTCCCACCAGGGCCAGCAGCAACCTTACAACCAGAGCCAGTACAGCAGCTACGGCACGCCACAGGGCAAGCAGAAAGGCTATGGCCATGGGCAGGGCAGCTACTCCTCCTACTCCAACTCTTACAACTCCCCAGGTGGTGGTGGGGGCTCTGACTACAGCTACGACAGCAAATTCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032178_MULTIEX2-1/2=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.056 A=0.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGGGTACAGCAGCAATTCGG
R:
TCCTTGGTAGTAGGAGCTGTAGC
Band lengths:
134-195
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa Enriched at 14 days (FC=1.278, FDR=0.002), PC9 No Change at 14 days (FC=0.979, FDR=0.749), Late Xenograft No change (FC=0.994, FDR=0.324).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types