DreEX0046175 @ danRer10
Exon Skipping
Gene
ENSDARG00000045803 | mfge8b
Description
milk fat globule-EGF factor 8 protein b [Source:ZFIN;Acc:ZDB-GENE-070209-137]
Coordinates
chr25:19066153-19080399:+
Coord C1 exon
chr25:19066153-19066284
Coord A exon
chr25:19077643-19077756
Coord C2 exon
chr25:19080218-19080399
Length
114 bp
Sequences
Splice sites
3' ss Seq
GACTCTTCTGTTTGTTTCAGATG
3' ss Score
11.41
5' ss Seq
TGCGTAAGT
5' ss Score
9.73
Exon sequences
Seq C1 exon
GCCCCTGCAACCCAAACCCCTGTAAAAATGATGGCGTGTGCGAGATCATCTCCAACTCTCGACGAGGAGACGTTTTCAGCGAATACATCTGCAAGTGTCCAGTGGGCTTCGAGGGAATCCACTGTCAAAACA
Seq A exon
ATGTGAATGACTGTGCGGGTCAGCCCTGTCTAAACGGAGGCACCTGTCAAGATCTTGATGGGGACTTCAACTGTAAATGTCCCTCGCCCTATGTTGGAAAACACTGCAATCTGC
Seq C2 exon
GATGCATCTCTCTGCTGGGAATGGAAGGAGGAGGAATTGCTGAGTCTCAGATCTCTGCATCTTCAGTGTACTATGGCATTCTGGGACTGCAGCGATGGGGACCCGAGTTGGCTCGGCTTAATAACAAGGGACTTGTTAATGCTTGGACCTCTGCAACGCATGACAAAAACCCCTGGATTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000045803-'3-4,'3-3,4-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0000822=EGF=WD(100=86.7),PF0000822=EGF=PU(7.9=6.7)
A:
PF0000822=EGF=PD(89.5=87.2)
C2:
PF0075420=F5_F8_type_C=PU(31.7=72.1)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Cow
(bosTau6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCAACTCTCGACGAGGAGAC
R:
TTTGTCATGCGTTGCAGAGGT
Band lengths:
249-363
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]