RnoEX0054079 @ rn6
Exon Skipping
Gene
ENSRNOG00000017510 | Mfge8
Description
milk fat globule-EGF factor 8 protein [Source:RGD Symbol;Acc:3083]
Coordinates
chr1:140854942-140857508:-
Coord C1 exon
chr1:140857377-140857508
Coord A exon
chr1:140855351-140855518
Coord C2 exon
chr1:140854942-140855123
Length
168 bp
Sequences
Splice sites
3' ss Seq
CTGCTTTTCTCTCCTACCAGAGA
3' ss Score
9.64
5' ss Seq
CCCGTGAGT
5' ss Score
8.4
Exon sequences
Seq C1 exon
GACCGTGTTCCCCAAACCCTTGCTTCCACGATGCCAAATGCCTGGTGACTGAGGACACACAGCGAGGGGACATCTTCACTGAGTACATCTGCCAGTGCCCTGTGGGCTACTCGGGCATCCACTGTGAACTCG
Seq A exon
AGACCACCTCCTACCTGGATGGAGAGTACCTGTCCAGCCCAGCCGTCCCTACCACAGCCGTCCCCACCACAGCCATCCCCACCACAGCCGTCCCCACCACAGCCGTCCCCACCACAGCCGTCCCCACCCCGGCCCCCAACCCCGATCTTTCCAACCACCTAGCCTCCC
Seq C2 exon
GCTGTTCCACAAAGCTGGGCTTGGAAGGGGGCGCCATTGCCGATTCACAGATTTCTGCCTCGTCTGTGTATATGGGCTTCATGGGCTTGCAGCGCTGGGGCCCGGAGCTGGCTCGCCTGTATCGCACAGGGATTGTCAATGCATGGACAGCCAGCAGCTATGATAGCAAGCCCTGGATCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000017510_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.421 C2=0.000
Domain overlap (PFAM):
C1:
PF0000822=EGF=WD(100=86.7)
A:
NO
C2:
PF0075420=F5_F8_type_C=PU(31.7=72.1)
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAATGCCTGGTGACTGAGG
R:
TGGCTGTCCATGCATTGACAA
Band lengths:
252-420
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]