DreEX0050008 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23015988-23019557:-
Coord C1 exon
chr9:23019453-23019557
Coord A exon
chr9:23016894-23016998
Coord C2 exon
chr9:23015988-23016092
Length
105 bp
Sequences
Splice sites
3' ss Seq
TATTGGCCATTCCTCTTTAGTAC
3' ss Score
5.09
5' ss Seq
CACGTAAGT
5' ss Score
10.16
Exon sequences
Seq C1 exon
GTGAAATACAAAGGTGACCTGAAGAAGATTCACAAGCCTGTTACAGACATGGCAGAATCTCTGTCCATGCAACACAACCTGAGCACTAGCAAGCTGGCCAGTGAT
Seq A exon
TACCAGTACAAGAAGAAGTTTGAGGCGAGTAGGGGTCACTACCATATGATACCCGACACACCTGAGCAGCTTCACCACAAAGAAGCCTCAGAGCTGCAGAGTCAC
Seq C2 exon
GTGAAGTATAAGGAGAAATATGAGAAAGAAAGAGGCAAAGCCATGCTGGACTTTGAGACTCCAACATATGTGACTGCTAAAGAGGCTCAGCATCTGCAGAGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX1-4/13=3-5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.377 A=0.686 C2=0.269
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(40.0=27.8),PF0088013=Nebulin=PU(55.2=44.4)
A:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
C2:
PF0088013=Nebulin=PD(41.4=33.3),PF0088013=Nebulin=PU(55.2=44.4)
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGAAATACAAAGGTGACCTGAAGA
R:
CTGCAGATGCTGAGCCTCTTT
Band lengths:
204-309
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]