DreEX0050010 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23011116-23016092:-
Coord C1 exon
chr9:23015988-23016092
Coord A exon
chr9:23015492-23015602
Coord C2 exon
chr9:23011116-23011229
Length
111 bp
Sequences
Splice sites
3' ss Seq
TCTATCTTTGCGGTGGATAGAAA
3' ss Score
2.75
5' ss Seq
GAGGTAACA
5' ss Score
8.1
Exon sequences
Seq C1 exon
GTGAAGTATAAGGAGAAATATGAGAAAGAAAGAGGCAAAGCCATGCTGGACTTTGAGACTCCAACATATGTGACTGCTAAAGAGGCTCAGCATCTGCAGAGTCAG
Seq A exon
AAAGAGTATAAGAAGGACTTTGAGATGACAATGAAAGGAAAGAACCTCTCTGGGTTGGAGGTCACCCCTGCCATGATGCATGTCAGACACGCCACCAAAATTGTTAGCGAG
Seq C2 exon
AGAGAGTACAAGAAAGCTTTGGAGCAAGAGATCAAGGGAAAAGGCATGTTGGCTTTGGCTACTGACACTCCAGACTTCTTGAGGGCAAGGAACGCCACAGAAATCCTCAGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX1-6/13=5-8
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.269 A=0.411 C2=0.077
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(41.4=33.3),PF0088013=Nebulin=PU(55.2=44.4)
A:
PF0088013=Nebulin=PD(41.4=31.6),PF0088013=Nebulin=PU(55.2=42.1)
C2:
PF0088013=Nebulin=PD(41.4=30.8),PF0088013=Nebulin=PU(55.2=41.0)
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGAAATATGAGAAAGAAAGAGGCA
R:
TGAGGATTTCTGTGGCGTTCC
Band lengths:
203-314
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]