DreEX0050011 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23011116-23015602:-
Coord C1 exon
chr9:23015492-23015602
Coord A exon
chr9:23013261-23013371
Coord C2 exon
chr9:23011116-23011229
Length
111 bp
Sequences
Splice sites
3' ss Seq
TGTTCTTCTCCGCCCTGTAGAAA
3' ss Score
9.74
5' ss Seq
GAGGTAGGA
5' ss Score
8.24
Exon sequences
Seq C1 exon
AAAGAGTATAAGAAGGACTTTGAGATGACAATGAAAGGAAAGAACCTCTCTGGGTTGGAGGTCACCCCTGCCATGATGCATGTCAGACACGCCACCAAAATTGTTAGCGAG
Seq A exon
AAAGAGTACAGAAGGGATCTAGAGGAAGGGGTGAAGGGTAAAGGGCTTACAGTGCTGGAGGAAACTCCTGAATTACTGAGAGCAAAGAATGCTACTCAGATCCTATGTGAG
Seq C2 exon
AGAGAGTACAAGAAAGCTTTGGAGCAAGAGATCAAGGGAAAAGGCATGTTGGCTTTGGCTACTGACACTCCAGACTTCTTGAGGGCAAGGAACGCCACAGAAATCCTCAGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX1-7/13=6-8
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.411 A=0.196 C2=0.077
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(41.4=31.6),PF0088013=Nebulin=PU(55.2=42.1)
A:
PF0088013=Nebulin=PD(41.4=31.6),PF0088013=Nebulin=PU(55.2=42.1)
C2:
PF0088013=Nebulin=PD(41.4=30.8),PF0088013=Nebulin=PU(55.2=41.0)
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTATAAGAAGGACTTTGAGATGACA
R:
GACTGAGGATTTCTGTGGCGT
Band lengths:
219-330
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]