DreEX0051735 @ danRer10
Exon Skipping
Gene
ENSDARG00000038991 | nsfb
Description
N-ethylmaleimide-sensitive factor b [Source:ZFIN;Acc:ZDB-GENE-050808-1]
Coordinates
chr12:22227083-22230791:-
Coord C1 exon
chr12:22230692-22230791
Coord A exon
chr12:22229125-22229164
Coord C2 exon
chr12:22227083-22227249
Length
40 bp
Sequences
Splice sites
3' ss Seq
AAATGCTCTCTGTGTCACAGAGA
3' ss Score
6.78
5' ss Seq
AAGGTACGT
5' ss Score
10.75
Exon sequences
Seq C1 exon
GCATTTGACTATTAAAACCACTCCAACTCAGAAATTTGTTTTCACTGTGAGAACTCATCCAAGCGTGGTGCCAGGAACCATTGCCTTCAGTCTACCTCAG
Seq A exon
AGAAAATGGGCTGGACTCTCTCTAAACCAAGATGTGGAAG
Seq C2 exon
TGAGTGTCTACAACTTTGACCCGTCCAGACAGTATGTGGGAACCATGACAATCGAGATCGACTTCCTCCAGAAAAAGAGCATTGACAGCAATCCTTATGACTCTGATAAAATGGCCATTGAATTTATTCAGTGTTTCACTGCACAGGCCTTTAGCATGAGTCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000038991_MULTIEX1-2/2=C1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.066 A=0.071 C2=0.000
Domain overlap (PFAM):
C1:
PF0235913=CDC48_N=FE(40.2=100)
A:
PF0235913=CDC48_N=FE(15.9=100)
C2:
PF0235913=CDC48_N=PD(7.3=10.7),PF0293312=CDC48_2=PU(36.9=42.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAACTCATCCAAGCGTGGTG
R:
TGCTCTTTTTCTGGAGGAAGTCG
Band lengths:
132-172
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]