MmuEX6090318 @ mm9
Exon Skipping
Gene
ENSMUSG00000034187 | Nsf
Description
N-ethylmaleimide sensitive fusion protein [Source:MGI Symbol;Acc:MGI:104560]
Coordinates
chr11:103777820-103790157:-
Coord C1 exon
chr11:103790058-103790157
Coord A exon
chr11:103787462-103787501
Coord C2 exon
chr11:103777820-103777986
Length
40 bp
Sequences
Splice sites
3' ss Seq
CTGTCCTTTTTATTTTATAGCGA
3' ss Score
10.4
5' ss Seq
AAGGTAGGT
5' ss Score
10.29
Exon sequences
Seq C1 exon
GCATGTGATGGTGAGGACGTCCCCCAATCACAAGTACATATTTACGCTGAGGACCCATCCGTCAGTGGTTCCAGGATGCATCGCGTTCAGCTTGCCTCAG
Seq A exon
CGAAAATGGGCTGGACTTTCTATTGGACAGGACATAGAAG
Seq C2 exon
TTGCCTTGTATTCGTTTGACAAAGCCAAGCAGTGCATCGGCACAATGACCATCGAGATCGACTTCCTGCAGAAGAAGAACATCGACTCCAACCCTTACGACACCGACAAGATGGCCGCCGAGTTCATCCAGCAGTTCAACAACCAGGCCTTCTCAGTGGGGCAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000034187-'4-6,'4-5,5-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.029 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0235913=CDC48_N=PU(58.8=96.8)
A:
PF0235913=CDC48_N=PD(16.0=85.7)
C2:
PF0235913=CDC48_N=PD(11.8=10.7),PF0293312=CDC48_2=PU(30.0=42.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCGTCAGTGGTTCCAGGAT
R:
TGTCGTAAGGGTTGGAGTCGA
Band lengths:
146-186
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: