DreEX0051775 @ danRer10
Exon Skipping
Gene
ENSDARG00000101234 | nsmfb
Description
NMDA receptor synaptonuclear signaling and neuronal migration factor b [Source:ZFIN;Acc:ZDB-GENE-080603-4]
Coordinates
chr5:27833078-27880202:+
Coord C1 exon
chr5:27833078-27833464
Coord A exon
chr5:27866292-27866371
Coord C2 exon
chr5:27880127-27880202
Length
80 bp
Sequences
Splice sites
3' ss Seq
GGTTTGTATGTTTTCTCCAGGGA
3' ss Score
7.56
5' ss Seq
GCAGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
AGAGCAAGTGCACGAGCGCATTCTGGCGTGCTGGCAGCTCACCGTCCACAATGCTCTGTTAACTCAAACTCTCTTCACCAGAGATCAAAAACTTACTTCTCGTAGTGAATACAGTCGGATGTGGCGTTCCTAAACATGAGGAACAAACGGAGCATCTGACACAGACCCTGAGCAGCAGTTTGATTCGGGGAGTAGAGTGAAGCCGATCGTATCTCCACTCTGCGGATGATTTGTTTGCGTTGTGCTTTTCTTTCCTTCATTGCAGATGTGGCGAGTGGAATGAGCTGTTTTCTCACATCGGTCTTGTCACTGCAGCATGGGAACTGCCGTGTCCAAAAAGAAGAATTTAAGGAATGATGCGATTTCTTCTGTTGCCGCAAAAGTTAG
Seq A exon
GGAGGCCCGGGTCATCTCTACCTTCTGAAGAACAAAGTGGCTACGTTTGCGAAAGTAGAGAAGGAGGAGGGCATGATGCA
Seq C2 exon
CTTTTTCAAAACCTCAAGAGACTGATGAAACCTCATGGCATTGAGTTCAAGTCTCCTCTGGAGCTGTCAGCACAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000101234_MULTIEX1-10/11=C1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.717 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF142521=DUF4347=FE(18.7=100)
C2:
PF142521=DUF4347=PD(7.9=42.3)
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACATCGGTCTTGTCACTGCA
R:
GTGCTGACAGCTCCAGAGGA
Band lengths:
167-247
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]