DreEX0054432 @ danRer10
Exon Skipping
Gene
ENSDARG00000013128 | pdk1
Description
pyruvate dehydrogenase kinase, isozyme 1 [Source:ZFIN;Acc:ZDB-GENE-091204-402]
Coordinates
chr9:3132113-3133563:-
Coord C1 exon
chr9:3133486-3133563
Coord A exon
chr9:3133240-3133316
Coord C2 exon
chr9:3132113-3132211
Length
77 bp
Sequences
Splice sites
3' ss Seq
GTGTGTGTTTTTTTCCACAGTTA
3' ss Score
11.18
5' ss Seq
AAGGTATTT
5' ss Score
7.64
Exon sequences
Seq C1 exon
ATGCTTATGAAAATGCCCGAAACCTGTGTGACCGGTATTATATGAACTCGCCCGAGCTAGTGTTGGAGGAATTTAATG
Seq A exon
TTAAAGGACCAGATAAACCCGTTACTGTGGTTTATGTACCATCTCATCTCTACCACATGGTGTTTGAGCTCTTTAAG
Seq C2 exon
AATGCCATGCGCGCTACCATGGAGCTTTATGAAGATGCCATGGAGTATCCCCCAGTGCATGTGCAGATCGTCCTCGGCCATGAAGACTTGACTGTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000013128-'5-6,'5-5,8-6
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0251821=HATPase_c=PU(11.2=53.8)
C2:
PF0251821=HATPase_c=FE(25.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGAAAATGCCCGAAACCTGTG
R:
ACAGTCAAGTCTTCATGGCCG
Band lengths:
167-244
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]