BtaEX0026436 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000016836 | PDK1
Description
pyruvate dehydrogenase kinase, isozyme 1 [Source:HGNC Symbol;Acc:HGNC:8809]
Coordinates
chr2:24092147-24095939:-
Coord C1 exon
chr2:24095862-24095939
Coord A exon
chr2:24094062-24094138
Coord C2 exon
chr2:24092147-24092245
Length
77 bp
Sequences
Splice sites
3' ss Seq
TTTTCTCTTCTGCTCTGTAGCAA
3' ss Score
8
5' ss Seq
AAGGTTTGT
5' ss Score
7.81
Exon sequences
Seq C1 exon
ATGGCTATGAAAATGCTAGGCGTCTGTGTGATTTGTATTATATTAACTCTCCCGAACTAGAACTTGAAGAACTAAATG
Seq A exon
CAAAATCACCAGGACAGCCAATACAAGTGGTTTATGTACCATCCCACCTCTATCACATGGTGTTTGAACTTTTCAAG
Seq C2 exon
AATGCAATGAGAGCAACGATGGAGCATCATGCTGACAAAGGTGTTTATCCGCCTATTCAGGTCCATGTCACACTGGGTAAAGAGGATTTGACTGTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000016836_MULTIEX3-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.037 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0251821=HATPase_c=PU(11.2=53.8)
C2:
PF0251821=HATPase_c=FE(25.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCTATGAAAATGCTAGGCGT
R:
CAAATCCTCTTTACCCAGTGTGACA
Band lengths:
167-244
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development