RnoEX0064562 @ rn6
Exon Skipping
Gene
ENSRNOG00000001517 | Pdk1
Description
pyruvate dehydrogenase kinase 1 [Source:RGD Symbol;Acc:69427]
Coordinates
chr3:58544151-58547148:+
Coord C1 exon
chr3:58544151-58544228
Coord A exon
chr3:58545525-58545601
Coord C2 exon
chr3:58547050-58547148
Length
77 bp
Sequences
Splice sites
3' ss Seq
GTTTCTCTTCTGCTCTGTAGCGA
3' ss Score
7.31
5' ss Seq
AAGGTGTGT
5' ss Score
6.64
Exon sequences
Seq C1 exon
ATGGCTATGAGAATGCGAGACGGCTTTGTGATTTGTATTACATTAACTCTCCTGAACTAGAACTTGAAGAACTAAATG
Seq A exon
CGAAATCACCAGGACAGCCAATACAAGTGGTTTATGTTCCGTCCCATCTCTATCACATGGTGTTTGAACTGTTCAAG
Seq C2 exon
AACGCAATGAGGGCCACCATGGAGCACCATGCAGACAAAGGCGTTTATCCCCCGATTCAAGTTCACGTCACACTGGGGGAGGAGGATTTGACTGTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000001517_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.074 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0251821=HATPase_c=PU(11.2=53.8)
C2:
PF0251821=HATPase_c=FE(25.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCTATGAGAATGCGAGACGG
R:
AGTCAAATCCTCCTCCCCCAG
Band lengths:
169-246
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]