DreEX0055013 @ danRer10
Exon Skipping
Gene
ENSDARG00000074303 | phf12a
Description
PHD finger protein 12a [Source:ZFIN;Acc:ZDB-GENE-030131-3876]
Coordinates
chr10:36873230-36874241:-
Coord C1 exon
chr10:36874098-36874241
Coord A exon
chr10:36873421-36874006
Coord C2 exon
chr10:36873230-36873346
Length
586 bp
Sequences
Splice sites
3' ss Seq
TTGCTTATGCTTTGGTATAGTGG
3' ss Score
4
5' ss Seq
GTGGTAAAT
5' ss Score
5.18
Exon sequences
Seq C1 exon
GTTCCAGATGCCATAAAGTCACAGTATCAGAACCCACCTACTTTGATGGCATCAGCAGGAGTCCGTGAAGGAGAACTGATCTGTACTGGCCTTCCTGAAGCTTCTGTCCCACAGCACTTAGCCAGTGAAGATGAACAACATCAG
Seq A exon
TGGCTCAAAGACATCATCTCGCTGCAGTGCAGCATTATGAGGCATTTAAGGAACAGACAAACATCTCATTGGGATTCAGAGCACACAGAAAAGACTGATCTAAAACTCAGAGACTCCTGCCAGAGTGCCGCCTCGCAGGGCAGCCAGAACTCTTCACCAAAAGTTCCCTGTAGCTCATGTACTGAGGGACTGTGTCAGAACTGTAAGACCACAAACGGCCCTGTAGACCAATCGGTTCAGTCCAATGGAACACAGGATCCTGTTAGAACCCAAAACAAGAGCGCTGACCGTGAAAAGCCCTGTTTACCCATGCCCAGTCCACTCAACCATGCCAACTCTGGAGCCATTATCAAGGGCGAGGATGCCAGTTTAAAGCCTCGTAGTGGCACTGATGTCCTTCCTCCTGCTCGTGTCAAATCTGAGCCCCGAACTCAACCATGTGATCATGCTAAAACTCCACCGGCTTCAGATGCAGCTGCAAAAGTCTCAGACTCCACTGTATGTACAGGAGCACATGCTGTTAAACCACAAAAAGTCACCTCAGACGCTGGGTCAAATGGACAGAGATCTCCATCTTCTTCTTGTG
Seq C2 exon
GTGGAAAGTCCAGTCTGGACTCAGTGATGGCAGGCAGTGGGGACATCTCCTCTTGCACAGAACCCAACATGTCAGAGCTGTCCGGTCGGATAAAAGACATCATGCAGGGCGGTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074303-'8-9,'8-8,9-9
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.396 A=0.827 C2=0.825
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCAGATGCCATAAAGTCACAGT
R:
CCGCCCTGCATGATGTCTTTT
Band lengths:
254-840
Functional annotations
There are 2 annotated functions for this event
PMID: 24782567
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: detection by mass spectrometry. ELM ID: ELMI002883; ELM sequence: EKADIKPV; Overlap: FULL
PMID: 25218447
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: Identification by mass spectrometry, detection by mass spectrometry, his tag, pull down. ELM ID: ELMI002883; ELM sequence: EKADIKPV; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]