DreEX0055016 @ danRer10
Exon Skipping
Gene
ENSDARG00000075509 | phf12b
Description
PHD finger protein 12b [Source:ZFIN;Acc:ZDB-GENE-110628-2]
Coordinates
chr15:24621840-24623224:+
Coord C1 exon
chr15:24621840-24621992
Coord A exon
chr15:24622069-24622627
Coord C2 exon
chr15:24623117-24623224
Length
559 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTTTTCTTTTCACAGTGG
3' ss Score
10.18
5' ss Seq
AAGGTATTA
5' ss Score
7.04
Exon sequences
Seq C1 exon
GTCCCCAATGCCATTAAGAGTCACTATCAAAATCCCCCTCTGCTGATGGATCAAGTGGGCCTTCGAAATGGTGAGCTGGTCCCAAACGGTTTCATGGAACAAGAATGTTCTTCATCTACCCAACATTTAACCAGTGAAGAGGAGCAAGAGGAG
Seq A exon
TGGCTTCGTCATGTCATTGCACTTCAGTGCAGTATTATGAAACATTTATCTGCTAAGCAGATGTCATCTTCCCTTCGGGACTCGGAGCAGGCTGATTTATCAGATGCAAAGACGCAGGAGTGTATGGAGATGCAGGAGCTCAGCTCTCAGGACTCTGATTACTTGCAGAGCCGCAGTACAAAAGTTGGCCCCCAGGGGGCCCAAGTGCTGTCCACTATTGTTCCCAAACATCCTGAGAGCTGCAATGAACAATCTAGTCAGGGTGAGGCTGACAGTCCAACAGAGAAGGGCACAGGAGTGAATGGGCCGGTGGCATGTGAAGGTGACACCTGTACGACCTCACACAAGCCCAGCAGCCCCTCTAGACGACAGAATCTTGAATCAGCCCTGCCCAACCATGTAGACATAAAGACTGAGAGCAGCAGTCCTTTCAGCTCCTGTATGCAGAGAGATAGTCCGTCTACCAGCCTGCTATCAGGCTCGCCTGCCCGTCATCATGGCTCAACTCTTGGGAACTCTTCCTCAGGGCTGAACAGCAGCTTGTCATCTCAAACTAAAG
Seq C2 exon
AGAACAAAGTGAGCCCTGACTTAATGTCTGCTGGAAAAGGCTCAACGCTCCCGTCTTCAATAGGCAGCTTATACAGCTACATCAAGAATGTGGTTCAAGGCGATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075509-'10-16,'10-14,11-16
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.314 A=0.904 C2=0.405
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCCAATGCCATTAAGAGTCACT
R:
TCGCCTTGAACCACATTCTTGA
Band lengths:
254-813
Functional annotations
There are 2 annotated functions for this event
PMID: 24782567
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: detection by mass spectrometry. ELM ID: ELMI002883; ELM sequence: EKADIKPV; Overlap: FULL
PMID: 25218447
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: Identification by mass spectrometry, detection by mass spectrometry, his tag, pull down. ELM ID: ELMI002883; ELM sequence: EKADIKPV; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]