DreEX6002508 @ danRer10
Exon Skipping
Gene
ENSDARG00000026855 | cacna2d4a
Description
calcium channel, voltage-dependent, alpha 2/delta subunit 4a [Source:ZFIN;Acc:ZDB-GENE-041210-213]
Coordinates
chr4:20568729-20569438:-
Coord C1 exon
chr4:20569276-20569438
Coord A exon
chr4:20569071-20569202
Coord C2 exon
chr4:20568729-20568789
Length
132 bp
Sequences
Splice sites
3' ss Seq
AATCTTTCCTTGTCACTTAGATC
3' ss Score
6.44
5' ss Seq
CAGGTAATC
5' ss Score
6.99
Exon sequences
Seq C1 exon
TTTGACTACTACAATTCCATGTTGATTAACACATTAAATGAAGATGGAAACAACATAGAGCTGGGGGGTGAATTTCCTTTGGAGGAGAATGAACACTTCAACAATCTGCCTGTCAATACGCAGCAAAGCGTCGTACAAGTGCCCACAAATGTTTATAACAAAG
Seq A exon
ATCCTGATATCCTGAACGGGGTGTTTATGTCAGAAGCACTGAATGACATATTTATCAGTAACTTTGAGAAGGACCCGACTCTAACCTGGCAGTACTTCGGCAGTTCAACTGGATTCTTTAGACTTTACCCAG
Seq C2 exon
GTATTAAGTGGACACCTGATGAAAATGGAGTTGTCACTTTTGATGGAAGGAATCGCAACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000026855-'5-4,'5-3,6-4=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.236 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF083996=VWA_N=FE(46.2=100)
A:
PF083996=VWA_N=FE(37.6=100)
C2:
PF083996=VWA_N=PD(3.4=19.0)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGACTACTACAATTCCATGTTGATT
R:
CAGTTGCGATTCCTTCCATCA
Band lengths:
223-355
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]