DreEX6011117 @ danRer10
Exon Skipping
Gene
ENSDARG00000061486 | DNAH10
Description
dynein, axonemal, heavy chain 10 [Source:HGNC Symbol;Acc:HGNC:2941]
Coordinates
chr5:27744230-27747004:+
Coord C1 exon
chr5:27744230-27744361
Coord A exon
chr5:27744442-27744658
Coord C2 exon
chr5:27746885-27747004
Length
217 bp
Sequences
Splice sites
3' ss Seq
AACATGTTTTTCTCCTGCAGGTG
3' ss Score
12.5
5' ss Seq
CAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GAAAAGGCAGATCTGTGTAAACTGTTTGAAAAGTATGTGCCGAGTGCCATTGATATGATAGTGGATGGTGTGGTTGATGGTAAACAGACAGAGAAGCTAAAGACTGTAATCCTGCAGACGGATTTAAACATG
Seq A exon
GTGATGCAGTTGACTGTTATGGTGGATTCTTTGCTGGAGAATGAGGATTTTCTTTTTGAGGAGCTGGAGTGTTGTTTCCTAGAGGCTCTCTACTGTTCACTGGGGGCTTCACTCCTCGATAAAGGCAGACAAAAGTTTGATGCTTTTATCAAAAAGCTGTCCTCTCTCAACAGTGTTCATGATGAGAAGGTGCTGGCTGGTCCTGGAGAGATTCCAG
Seq C2 exon
TTTACTTGCCGACTCTCTATGATTTTCATTTTGATGGAACCCAAAAGAAGTGGGTTCCCTGGAGCTCCATGGTCTCAAAATACATTCACAATCCTGAAATGAAATTCATCGACATCCTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061486-'47-44,'47-43,48-44=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.007 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF127752=AAA_7=PU(1.1=4.1)
C2:
PF127752=AAA_7=PU(4.8=31.7)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAAAGGCAGATCTGTGTAAACTGT
R:
CAAGGATGTCGATGAATTTCATTTCA
Band lengths:
252-469
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]