DreEX6011827 @ danRer10
Exon Skipping
Gene
ENSDARG00000003592 | surf1
Description
surfeit 1 [Source:ZFIN;Acc:ZDB-GENE-041108-2]
Coordinates
chr5:32681261-32682190:+
Coord C1 exon
chr5:32681261-32681339
Coord A exon
chr5:32681832-32681959
Coord C2 exon
chr5:32682108-32682190
Length
128 bp
Sequences
Splice sites
3' ss Seq
AAAATTGTGTTTCATTTTAGATA
3' ss Score
9.11
5' ss Seq
CAGGTACAG
5' ss Score
8.68
Exon sequences
Seq C1 exon
GCTCTCTTTTCACCAAGGAGGACTCTGTTTGTGACACGACAGAGTTTATTCAGACGTCAGGATGCTCCAGTGCTCCGGT
Seq A exon
ATAACCTGGCCAGGTTTAGTCAACAATCAACTTCCACTGTCGAAGGAACAAAGAAAGGAGAAGACACCTTTCTGAAGTGGTTTCTCTTGTTGATACCCGTCACCACCTTTTGTCTTGGAACATGGCAG
Seq C2 exon
GTGAAACGTAGAAAGTGGAAGTTGGAGCTGATCAGTGAACTTCAGAGTCTGACGACTTCAGTGCCAATCCCACTGCCTGTAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000003592-'2-3,'2-2,3-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.023 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0210410=SURF1=PU(6.4=32.6)
C2:
PF0210410=SURF1=FE(12.3=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTCTCTTTTCACCAAGGAGGA
R:
TCTACAGGCAGTGGGATTGGC
Band lengths:
162-290
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]