DreEX6013873 @ danRer10
Exon Skipping
Gene
ENSDARG00000029372 | rasa4
Description
RAS p21 protein activator 4 [Source:ZFIN;Acc:ZDB-GENE-060526-349]
Coordinates
chr5:58698767-58710887:+
Coord C1 exon
chr5:58698767-58698911
Coord A exon
chr5:58708551-58708723
Coord C2 exon
chr5:58710750-58710887
Length
173 bp
Sequences
Splice sites
3' ss Seq
TTACTTTATTTTGAATCCAGATC
3' ss Score
6.08
5' ss Seq
AAGGTGGGC
5' ss Score
7.93
Exon sequences
Seq C1 exon
GCTGTGCAGACCATTGGGAACATGGACACTCTGGCCTGCTGCTCTAAAGAGCCGTGGATGGTGCGTTTGCAGCCTGCCATTCAGCAAGGCATCACCCAGCTCAAAGACTTCATCACCAGACTGGTCAACTGCCATGAGTCAGAGG
Seq A exon
ATCTTGAACTTCAGACACGAATGAGCCTCCAGTGCGGCACTATGGAGAAAGAGGGTTTTCTCTTCCTGCACAGGACCAAGGACAAGTGTATGCCAATGACTTCTCCCTTTAAGAAGTACTATGTGACGCTCAGTAAGGACACGCTGTCTTATTCCAGGACTCTGCACTCTAAG
Seq C2 exon
AAGAGCTCATCTATTTCCCTGCCAAAGATCCGTGCGGTAGAGAAGGTGGAGGAGAAGTGTTTCGGCAGTGCCAACGTCATGCAGATCATCTCCAGTGAAGACTCAGGCCAGCAGGAGACCCTATACCTCGACTGCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000029372-'16-18,'16-17,17-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=PD(3.2=12.2)
A:
PF0016924=PH=PU(40.2=74.1)
C2:
PF0016924=PH=FE(42.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCAGACCATTGGGAACATGG
R:
GGCCTGAGTCTTCACTGGAGA
Band lengths:
250-423
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]