MmuEX6021646 @ mm10
Exon Skipping
Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136103280-136104653:+
Coord C1 exon
chr5:136103280-136103424
Coord A exon
chr5:136103837-136104006
Coord C2 exon
chr5:136104516-136104653
Length
170 bp
Sequences
Splice sites
3' ss Seq
ATTTTCTTTCCTGCTTTCAGAGC
3' ss Score
11.04
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GCGGTCCAGAACATAGGCAATATGGACACACCGGTCTCTAGGGCCAAAGAGTCGTGGATGGAACCACTCCAGCCTACCGTGCGCCAGGGCGTGGCGCAGCTGAAGGACTTCATTATGAAGCTTGTGGACATAGAAGAAAAGGAAG
Seq A exon
AGCTGGACCTGCAGCGGGCTTTGAACTCACAGGCACCACCTGTGAAGGAGGGACCATTGTTCATCCACAGGACCAAAGGCAAAGGCCCTCTTGCATCCTCCTCGTTCAAGAAACTCTACTTCTCCCTTACCACTGAAGCCCTCAGCTTCGCCAAGACGTCCAGCTCCAAG
Seq C2 exon
AAAAGCACCTTCATCAAGCTGGCCAGCATCCGGGCTGCGGAGAAGGTGGAGGAAAAGAGCTTCGGCAGCTCCCACATCATGCAGGTCATCTATGCAGATGATGTTGGCCGTGCCCAGACTGTCTACCTGCAGTGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952-'35-45,'35-34,36-45=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.163 A=0.082 C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=PD(3.2=12.2)
A:
PF0016924=PH=PU(40.2=75.4)
C2:
PF0016924=PH=FE(42.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAGGGCCAAAGAGTCGTGGAT
R:
TGCACTGCAGGTAGACAGTCT
Band lengths:
243-413
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types