DreEX6020122 @ danRer10
Exon Skipping
Gene
ENSDARG00000075924 | RAPGEF4 (1 of 2)
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr6:6743453-6745500:+
Coord C1 exon
chr6:6743453-6743636
Coord A exon
chr6:6745263-6745347
Coord C2 exon
chr6:6745440-6745500
Length
85 bp
Sequences
Splice sites
3' ss Seq
GTCCCTCGCTCTAATTTTAGGCC
3' ss Score
9.98
5' ss Seq
ACTGTAAGA
5' ss Score
4.8
Exon sequences
Seq C1 exon
TCGACCATGAACTCAACTTTCAAGATAAGTACCTCTTTTACCGTTTCCTCGACGATGAGGAGGAGGATGCGGTGTTGCCTAGCGACGATGAGAAACGCGAAGCTGAGGAAGAGCTGCAGGAGACACTCCTCTTCCTCTCTCAGATTGGCCCTGACGCGCACATGCGCATGATCCTCAGGAAACC
Seq A exon
GCCTGGTCAAAGAACAGCTGAAGACCTGGAGATTATTTATGATGAGCTGCTGCATATCAAAGCTGTCTCTCACCTGTCCAACACT
Seq C2 exon
GTGAAGAGAGAGCTCGCCGGTGTCCTCATCTTTGAGTCACACGCCAAAGCAGGCACTGTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075924-'11-13,'11-12,12-13=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.145 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061016=DEP=PD(21.1=24.2)
A:
NO
C2:
PF0002724=cNMP_binding=PU(22.5=42.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCGACGATGAGGAGGAGGATG
R:
TGGCGTGTGACTCAAAGATGA
Band lengths:
182-267
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]