DreEX6029952 @ danRer10
Exon Skipping
Gene
ENSDARG00000006526 | fn1b
Description
fibronectin 1b [Source:ZFIN;Acc:ZDB-GENE-030131-6545]
Coordinates
chr1:4828828-4831045:-
Coord C1 exon
chr1:4830933-4831045
Coord A exon
chr1:4829097-4829265
Coord C2 exon
chr1:4828828-4829004
Length
169 bp
Sequences
Splice sites
3' ss Seq
TAACTTCTTCCATTTGACAGAAA
3' ss Score
5.82
5' ss Seq
CTCGTATGT
5' ss Score
5.29
Exon sequences
Seq C1 exon
CTCCTGTTCGGGTGATCATCACTGAAGCTGGAAATCAGCCCAATTCCCATCCAATTCAATGGAACGCTCCTCCGTCTGCCCACATTACGCAATACATCCTGAAGTGGAGAGTC
Seq A exon
AAAAATACACGCACACCATGGAAGGAGGTCACTATTCCTGGCCACATCAACTCCTACACCATTTCAGGCCTCAAAGCAGGTTTGACGTATGAAGGCCAGTTGATCAGCATTCTGCGCTACGGACCAAGAGAGGTTACTCGGTTTGACTTCACCACCACATATGGCTCTC
Seq C2 exon
TGGCCAAAGCAGAGGGTGAGACCACCCAGCCTCGACAAGTAGTGGACACCTCAGAGTCTGTTACTGAAATTACCTCCAACAGCTTTGTCATCTCCTGGGTATCTGCATCTGACACAGTGTCTGGATTTAGAGTTGAATATGAACTCTCAGAAGAAGGAGCTCAGAAACGTGTGCTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000006526-'14-17,'14-16,15-17=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.368 A=0.000 C2=0.329
Domain overlap (PFAM):
C1:
PF0004116=fn3=FE(45.1=100)
A:
PF0004116=fn3=PD(51.2=73.7)
C2:
PF0004116=fn3=PU(56.4=73.3)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAGCCCAATTCCCATCCAAT
R:
GCACACGTTTCTGAGCTCCTT
Band lengths:
253-422
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]