DreEX6032876 @ danRer10
Exon Skipping
Gene
ENSDARG00000060184 | pkn1a
Description
protein kinase N1a [Source:ZFIN;Acc:ZDB-GENE-061207-17]
Coordinates
chr1:45126573-45131176:+
Coord C1 exon
chr1:45126573-45126767
Coord A exon
chr1:45129189-45129298
Coord C2 exon
chr1:45131033-45131176
Length
110 bp
Sequences
Splice sites
3' ss Seq
TTGTGTGTGTGTGTGTATAGCGG
3' ss Score
7.11
5' ss Seq
AGAGTGAGT
5' ss Score
7.36
Exon sequences
Seq C1 exon
GTCGGCTCCAGGTGACATTATTGGGCTGTATGGGTCTTCTGGAGGTGGTTCCCGGACGCTCTCGTGGTAGCCAGGTGGTTTTACCGTGTTACAGTCCAGGAGAGGGACGATCATTCATGCGCAGTGGAAAAGGCCTGTATAGTCGCAGTGGCAGCGTTAGTGGAAAAACACCCCTCAAAACCGACGAACTCTCCT
Seq A exon
CGGATGTGAGCGCAGTATTGAAGCTGGACAACACTGTGGTGTGTCAGACGGCATGGAAGATGGTTGGAGAACAGGGCTGGGATCAGACCTTCATCATCGAGCTGGAGAGA
Seq C2 exon
TCCAGAGAGATGGAGATTGCCGTGTACTGGAGAGACTACCGCTCTCTGTGTGCGCTGAAATTTCTCAAGCTGGAGGATTTCTTGGACAACCAGAAACACAAAGTACAACTAGAGCTAGAACCACAGGGACTGCTGCTGGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000060184-'9-11,'9-10,10-11=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.045 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGCTGTATGGGTCTTCTGGA
R:
CCAGCTTGAGAAATTTCAGCGC
Band lengths:
246-356
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]