DreEX6036469 @ danRer10
Exon Skipping
Gene
ENSDARG00000062338 | zeb2a
Description
zinc finger E-box binding homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-070912-553]
Coordinates
chr9:26019486-26022549:+
Coord C1 exon
chr9:26019486-26019594
Coord A exon
chr9:26019919-26021882
Coord C2 exon
chr9:26022369-26022549
Length
1964 bp
Sequences
Splice sites
3' ss Seq
TTTATTATTTCTTTTTAAAGGTG
3' ss Score
9.31
5' ss Seq
CAGGTATGT
5' ss Score
9.8
Exon sequences
Seq C1 exon
CCTCAACTGCTAAATGAAGGGGCTGGCAATCGCAAATTTAAATGCACAGAGTGCGGCAAAGCCTTTAAATACAAGCATCATTTGAAGGAGCACCTCCGGATTCACAGTG
Seq A exon
GTGAGAAGCCATATGAGTGCTCCAACTGCAAGAAGCGTTTCTCCCACTCTGGCTCCTACAGCTCCCACATAAGTAGCAAGAAGTGCATTGGCCTTATCTCCATTAATGGACGGGTGCGCCATGGAGTCAACAACAAGCCTGGCTCATCACCAAACTCTGCTGCATCCTCTCCTGGCAGTCCTGCCTTAGCTCAGCTCCGTCACAAACTGGAGAACGGCAGGTCCATGAGCCTCCAAGACCCATCCGCTCACACAGACATCAAGTCTGAACCCATGGACTTCAATGAGTACCGTCTCATGATTGCATCTCAACAAGAATATGGTGCGTCGGGAGCCTTTCTCAATGGAGGTGGCCGTGGAGGTAGTCCTCCGGGGATGCACAGCTCATCTCAGAACCCATTGCAACATCTGGGTATCGGGTCAGATTCTCATCCACTGGGCTATACAGGGTTCATCAACAACATGAGTGAGGTTCAGAAGGTGCTTCAGATTGTGGACAACACAGTTTGCAGGCAAAAAATGGATGGCAACCCTGAAGAGATCTCTAAGCTTCGAGCATACATGAAGGAATTAGGCTCCCAGATGGAGGAGCAGAATCGGGTACTGGCCTCACAGCAAAGCTTCCTTGGCGTTGGTCACAACAGTCCCACCAAGACCATCATTGACTACACACTGGAAAAAGTCAATGAAGCCAAAGCATGTCTCCAGAGCTTAACAGAAGATTCCAAGAGGCGGCTCATGGATATCAAGAAAGAAAGACCAAGCCACGCCATGGATCTTCTATCTGAAGACAAAGCACTGGAGAGGGATGCACAGTACGCACCTTTCTCTTGTCAGTATTGCAAAGAGACATTTTCTGGCCCTATTCCATTACATCAACACGAGCGCTATCTCTGCAAGATGAACGAGGAGATCAAGGCTGTCCTCAAACCTAATGATACTGTACCAACTGGTCGCAGGGGGCTTTTCGGCTCAGAGCAGCAGGCTGGTGTGATCTCCTCATCCCTCGAGAGGAATGCCACCAGCCCAGTCAACCCCTACAAGGATCACATGTCATTACTGAACGTCTACTTTTCCATGAACACTGAACCCAACTCAGAGGAACTGAGGAAGATCTCTATGGCAGTGGGTCTTCCTCAGGAGTTTGTGAAGGATGCATTTGTTCAATGGAAAGCCCAATCTCACCATAGCTTCTCTAGGAAAAGATCTCCTCCTCCTGAGCGAAGTGGAGAGACCAACCATGTCAGGGATTCAGCATTTGCCCGGTCACCGGTTTCTTTGGGGCAATATGGCGACTCAACAGCAGAGATCCAAGCCATCACCAATGGGGATTCAGGGCACAAGTTGTCCAGAACACATCAGATTACAGGCACCAGGCAGACCAATGAAAAGCCACTTGACTCTGTGGACCACTTGAGAGGGGAGACACCCTCACCTTTGAACCTCTCATCATCATCATCCAAACACTCACATAGCAGTTCATACACGCCTAACAGCCTCACCTCTGAGGATGCCCACGGGGAACCACTAGACTTGTCTTTGCCAAAACAAGTTTCCAAGGCAGAACGGAGGGCCAAACCGAATGGTTTTTCTATTGAACACACTAGTAATTCCACTGCACGTGAACCAGGAACAGAGCCTTTAAATTTGGCCCACATCAAGAAGGAGTTCAATGGACCCAACAGTTTGGGCAATGAAAATCAGATGGATAAAAGCTCAAGTCCCATCTTTAGCATAAACCCCTTTGGTGGAGGCCACATGTACACCTCTCTTCCACCACATGGCGCATTCCCTCCACCCACTTTCATGTCAACGACTCAAGCCAGCATACCGGGTCTAAGGCCTTACCCAGGGCTGGATCCCATGAGTTTTCTACCACCTATGGCTTATACTTACGCAGCAGGAGCAGCCACCTTTGCTGAGATGCAGCAACGTCGGAAATACCAGCGGAAACAAGGCTTCCAG
Seq C2 exon
GGGGATCTACTGGACAGTGCTGGGGACTATCTGTCAGGGCTAGAGGACCTGACAGACAGCGAGTCGCTTCTCGCCCGGAAGAAGATTAAGAAGACTGAAAGTGGTATGTACGCGTGTGACTTGTGCGACAAAACATTCCAGAAGACCAGTTCCCTCCTAAGACACAAATATGAGCACACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062338-'18-14,'18-12,19-14=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.216 A=0.602 C2=0.170
Domain overlap (PFAM):
C1:
PF134651=zf-H2C2_2=PU(38.5=27.0)
A:
PF134651=zf-H2C2_2=PD(57.7=2.3),PF0043516=Spectrin=WD(100=15.1)
C2:
PF134651=zf-H2C2_2=PU(38.5=16.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACTGCTAAATGAAGGGGCTGG
R:
CTGTGTGCTCATATTTGTGTCTT
Band lengths:
286-2250
Functional annotations
There are 3 annotated functions for this event
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000260; ELM sequence: PLNLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000261; ELM sequence: PLDLS; Overlap: FULL
PMID: 12714599
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning. ELM ID: ELMI000262; ELM sequence: PLNLT; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]