DreEX6044452 @ danRer10
Exon Skipping
Gene
ENSDARG00000057025 | chrna2b
Description
cholinergic receptor, nicotinic, alpha 2b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-041001-99]
Coordinates
chr20:19916882-19919689:+
Coord C1 exon
chr20:19916882-19917033
Coord A exon
chr20:19919379-19919423
Coord C2 exon
chr20:19919580-19919689
Length
45 bp
Sequences
Splice sites
3' ss Seq
CTTATTTCTCTCCTCCCCAGGAC
3' ss Score
11.59
5' ss Seq
CAGGTATGT
5' ss Score
9.8
Exon sequences
Seq C1 exon
TTTCTTGTTGTGAGAAAACACATTCTCATGCTGAAGATGAACTCTTTAAAAAGTTATTCGATGGCTATAACAAGTGGTCAAGGCCTGTACCCAACACCTCTGATGTTGTGATTGTCAAGTTCGGTTTATCCATCGCCCAGCTGATTGATGTG
Seq A exon
GACGAAAAGAATCAGATGATGACAACAAACGTCTGGCTCAAGCAG
Seq C2 exon
GAATGGAATGATTATAAACTGCGCTGGAAGCCTTCAGACTACGACAATGTGACTTCCATCAGGGTTCCATCAGAACTCATCTGGGTTCCGGATATAGTTTTGTATAACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000057025-'2-2,'2-1,3-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=PU(18.8=76.5)
A:
PF0293118=Neur_chan_LBD=FE(6.8=100)
C2:
PF0293118=Neur_chan_LBD=FE(17.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCAAGGCCTGTACCCAACAC
R:
TGGAACCCTGATGGAAGTCACA
Band lengths:
145-190
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]