Special

MmuEX6042225 @ mm10

Exon Skipping

Gene
Description
cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) [Source:MGI Symbol;Acc:MGI:87886]
Coordinates
chr14:66143357-66146515:+
Coord C1 exon
chr14:66143357-66143508
Coord A exon
chr14:66143921-66143965
Coord C2 exon
chr14:66146406-66146515
Length
45 bp
Sequences
Splice sites
3' ss Seq
TTCTATCTCTGCTACCCCAGGAT
3' ss Score
6.2
5' ss Seq
CAGGTGAGT
5' ss Score
10.67
Exon sequences
Seq C1 exon
TGTTGGCCCAGCAAGGCTCGCACACCCATGCCGAGGACCGCCTGTTCAAACACCTCTTTGGAGGCTACAATCGCTGGGCACGGCCAGTGCCCAACACTTCCGATGTGGTCATCGTGCGCTTTGGACTATCCATTGCACAGCTCATAGATGTG
Seq A exon
GATGAGAAGAATCAAATGATGACCACCAATGTCTGGCTAAAGCAG
Seq C2 exon
GAATGGAATGACTACAAGCTGCGCTGGGACCCGGCTGAGTTTGGCAACATCACCTCCCTCCGCGTCCCCTCAGAGATGATCTGGATCCCAGACATTGTTCTCTACAACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000022041-'1-7,'1-4,5-7=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact

Alternative protein isoforms (Ref)

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):

C1:
PF0293118=Neur_chan_LBD=PU(18.8=76.5)
A:
PF0293118=Neur_chan_LBD=FE(6.8=100)
C2:
PF0293118=Neur_chan_LBD=FE(17.4=100)


Main Inclusion Isoform:


Main Skipping Isoform:
NA


Other Inclusion Isoforms:


Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
CCCAACACTTCCGATGTGGTC
R:
AGATCATCTCTGAGGGGACGC
Band lengths:
145-190
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types