DreEX6044453 @ danRer10
Exon Skipping
Gene
ENSDARG00000057025 | chrna2b
Description
cholinergic receptor, nicotinic, alpha 2b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-041001-99]
Coordinates
chr20:19894037-19919423:+
Coord C1 exon
chr20:19894037-19894605
Coord A exon
chr20:19916882-19917033
Coord C2 exon
chr20:19919379-19919423
Length
152 bp
Sequences
Splice sites
3' ss Seq
TCTTGTTTTTCCTCTTGCAGTTT
3' ss Score
11.09
5' ss Seq
GTGGTAAGC
5' ss Score
8.15
Exon sequences
Seq C1 exon
GAGCCCGGTCAGGAGTGTAAACCTGCACTTTCTGACATAAATGGAGTCCCTTTGGAAAATATCTGCTTCTATATCAATACTTATTTTTGAATGGACGAGGAAATAAATACTTTTGCAGCACGGAATCTGTGTTTTTGATCTTCAGCTGAACAGGTGCGCCAAACTAAAGATTCTCAACCTGCCTGTTCCCTCTTTTTTGTACGGAACTTCCGTGTTGGAATAACAAGAATGGATAAACACTGGATTTATGCAACTTAATTCAAAATATATTTACGAAAAACACTTGAAAAGAACATCTCACCTTAAATATTACAACCAGCGGGTTGAACAACAGGAAAAATAGAAGTCATGAAAACAACTGAATTATACGGAAACTGGAGCGTGGAATCCAGCTGAAAATAGAAATGTTTTGAAAGTGTTTGGCTGTATAATGGCAAGCTTTACGAATCAGCTGGTCTTTCTGAGAATTACCCTTCTCTGTACTATCATATGTGAGTCAA
Seq A exon
TTTCTTGTTGTGAGAAAACACATTCTCATGCTGAAGATGAACTCTTTAAAAAGTTATTCGATGGCTATAACAAGTGGTCAAGGCCTGTACCCAACACCTCTGATGTTGTGATTGTCAAGTTCGGTTTATCCATCGCCCAGCTGATTGATGTG
Seq C2 exon
GACGAAAAGAATCAGATGATGACAACAAACGTCTGGCTCAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000057025-'1-1,'1-0,2-1=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(18.8=76.5)
C2:
PF0293118=Neur_chan_LBD=FE(6.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACCTTAAATATTACAACCAGCGGG
R:
TGCTTGAGCCAGACGTTTGTT
Band lengths:
246-398
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]