DreEX6050301 @ danRer10
Exon Skipping
Gene
ENSDARG00000011672 | sema3b
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [Source:ZFIN;Acc:ZDB-GENE-081105-78]
Coordinates
chr8:26572014-26574242:-
Coord C1 exon
chr8:26574098-26574242
Coord A exon
chr8:26572190-26572409
Coord C2 exon
chr8:26572014-26572102
Length
220 bp
Sequences
Splice sites
3' ss Seq
TGTCTCTCCCTCTTCCTCAGTGT
3' ss Score
10.05
5' ss Seq
CAGGTACAG
5' ss Score
8.68
Exon sequences
Seq C1 exon
TAGTGTATTTCAAGGTTCTGCAGTGTGTCTCTATACTATGAACGACATTCGCCGAGCATTTTTAGGACCTTTTGCCCATAAAGAGGGACCCAATTACCAGTGGATTCCATTCCAGGGTAAAGTGCCTTACCCACGGCCAGGCATG
Seq A exon
TGTCCCAGTAAGACCTTCGGCAGTTTCGAGTCCACCAAAGGTTTTCCTGATAACGTAATCCAGTTTGCACGGCACCATCCGCTAATGTTTAATCCAGTGACTCCTCTGGGTGGCCGACCGCTGTTCCTGCGCACCGGTATCCCATACACCTTCACTCAGATTACTGTAGACAGAGTCAATGCAGCCGACGGACACTATGATGTCATGTTCATCGGCACAG
Seq C2 exon
ATGTGGGCTCTGTGCTGAAGGTGATCTCGGTGCCCAAGGGAAGCTGGAGCAACACTGAGCTGCTGCTGGAGGAGCTGCACGTCTTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011672-'11-16,'11-15,12-16=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(10.9=100)
A:
PF0140314=Sema=FE(16.5=100)
C2:
PF0140314=Sema=FE(6.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTTCAAGGTTCTGCAGTGTGTCT
R:
CTTAAAGACGTGCAGCTCCTCC
Band lengths:
228-448
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]