DreEX6062545 @ danRer10
Exon Skipping
Gene
ENSDARG00000007219 | actn1
Description
actinin, alpha 1 [Source:ZFIN;Acc:ZDB-GENE-081113-4]
Coordinates
chr13:36207121-36209248:-
Coord C1 exon
chr13:36209108-36209248
Coord A exon
chr13:36207598-36207780
Coord C2 exon
chr13:36207121-36207255
Length
183 bp
Sequences
Splice sites
3' ss Seq
TGTCTGTGTGTGTGTTGCAGGGT
3' ss Score
11.63
5' ss Seq
AAGGTTTGA
5' ss Score
5.13
Exon sequences
Seq C1 exon
AGAACTGAGAAACTGTTGGAAACTATTGATCAGCTTTACCTGGAGTTCGCCAAAAGAGCAGCGCCCTTTAATAACTGGATGGAGGGAGCAATGGAGGACCTGCAGGACACTTTTATAGTCCACACCATTGAGGAAATACAG
Seq A exon
GGTCTCAGCACAGCCCATGAGCAGTTTAAAGCCACACTGCCTGAGGCAGATAAGGAGCGACAGGCCATCCTGGGCATCCATAATGAAATTGCTAAAATTGTGCAGACATACCACGTCAACATGGCTGGCCAAAACCCCTACACCACCATCAACCCCCAGGAGATCAATGCCAAGTGGGATAAG
Seq C2 exon
GTGAGGCAGTTGGTGCCCCAGAGAGATCAGGCCCTGATAGAGGAACACGCTCGTCAGCAGAATAATGAGAGGCTGCGCAGACAGTTTGCTAATCAGGCTAATGTTATCGGGCCCTGGATCCAGACCAAGATGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000007219-'19-19,'19-18,20-19=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.230 C2=0.356
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(0.1=0.0),PF0043516=Spectrin=PU(32.1=76.6)
A:
PF0043516=Spectrin=FE(53.6=100)
C2:
PF0043516=Spectrin=PD(11.6=28.9),PF0043516=Spectrin=PU(20.2=46.7)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGCTTTACCTGGAGTTCGCC
R:
ATCTTGGTCTGGATCCAGGGC
Band lengths:
242-425
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]